| Literature DB >> 17353016 |
Masayo Kimura-Matsumoto1, Yukio Ishikawa2, Kazuo Komiyama3, Tadashi Tsuruta3, Makoto Murakami4, Seiko Masuda5, Yoshikiyo Akasaka2, Kinji Ito2, Shigeki Ishiguro2, Hiroshi Morita6, Shinji Sato6, Toshiharu Ishii2.
Abstract
Secretory phospholipase A2s (sPLA2s) contribute to the hydrolysis of phospholipid. Among them, sPLA2-IIA, -V, and -X have been regarded as enhancers of lipid accumulation in arterial intima. However, the distribution and production of the other types of sPLA2 in human aortic wall remain unclear. Therefore, in this study, the distribution and production of seven types of sPLA2 including IIA, IID, IIE, IIF, III, V, and X in atherosclerosis development in the human aorta were comprehensively examined by immunohistochemistry and in situ hybridization (ISH). The extent of sPLA2s expression increased with atherosclerosis development, but only sPLA2-IIF was never observed in the normal aorta. Double-immunostaining demonstrated that sPLA2-V expression was limited to smooth muscle cells (SMCs), although the other sPLA2s were expressed in both macrophages and SMCs. ISH using sPLA2 cDNAs revealed that the expression pattern of each mRNA was consistent with the results of immunohistochemistry for each corresponding sPLA2. These results indicate that the seven types of sPLA2 are expressed with various patterns in all stages of atherosclerosis development and may play an atherogenic role through degradation of phospholipid.Entities:
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Year: 2007 PMID： 17353016 DOI： 10.1016/j.atherosclerosis.2006.08.062
Source DB: PubMed Journal: Atherosclerosis ISSN： 0021-9150 Impact factor: 5.162