Literature DB >> 1735128

One-step transformation of yeast in stationary phase.

D C Chen1, B C Yang, T T Kuo.   

Abstract

A fast yeast-transformation technique has been developed by adding thio compounds to alkali-ion based protocols and incubating at 45 degrees C. This procedure is especially recommended for cells from stationary phase at a density up to 2.5 x 10(8) cells/ml. It involves only one step for the preparation and transformation of competent cells within 30 min. The yield was more than 10(4) transformants/microgram plasmid DNA. This protocol is easy to scale up for many DNA samples and is also applicable for yeast cells from different types of storages.

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Year:  1992        PMID： 1735128     DOI： 10.1007/bf00318659

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  8 in total

1.  Manipulating yeast genome using plasmid vectors.

Authors:  T Stearns; H Ma; D Botstein
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

2.  Factors enhancing genetic transformation of intact yeast cells modify cell wall porosity.

Authors:  B Brzobohatý; L Kovác
Journal:  J Gen Microbiol       Date:  1986-11

3.  Proteinase mutants of Saccharomyces cerevisiae.

Authors:  E W Jones
Journal:  Genetics       Date:  1977-01       Impact factor: 4.562

4.  Transformation of yeast.

Authors:  A Hinnen; J B Hicks; G R Fink
Journal:  Proc Natl Acad Sci U S A       Date:  1978-04       Impact factor: 11.205

5.  Transformation of intact yeast cells treated with alkali cations.

Authors:  H Ito; Y Fukuda; K Murata; A Kimura
Journal:  J Bacteriol       Date:  1983-01       Impact factor: 3.490

6.  High efficiency transformation of intact yeast cells by electric field pulses.

Authors:  E Meilhoc; J M Masson; J Teissié
Journal:  Biotechnology (N Y)       Date:  1990-03

7.  Saccharomyces cerevisiae mannoproteins form an external cell wall layer that determines wall porosity.

Authors:  H Zlotnik; M P Fernandez; B Bowers; E Cabib
Journal:  J Bacteriol       Date:  1984-09       Impact factor: 3.490

8.  A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

Authors:  R S Sikorski; P Hieter
Journal:  Genetics       Date:  1989-05       Impact factor: 4.562
  8 in total
  269 in total

1.  Characterization of the Saccharomyces cerevisiae cyclic nucleotide phosphodiesterase involved in the metabolism of ADP-ribose 1",2"-cyclic phosphate.

Authors:  F Nasr; W Filipowicz
Journal:  Nucleic Acids Res       Date:  2000-04-15       Impact factor: 16.971

2.  The numbers of individual mitochondrial DNA molecules and mitochondrial DNA nucleoids in yeast are co-regulated by the general amino acid control pathway.

Authors:  D M MacAlpine; P S Perlman; R A Butow
Journal:  EMBO J       Date:  2000-02-15       Impact factor: 11.598

3.  Maintenance of mating cell integrity requires the adhesin Fig2p.

Authors:  Mingliang Zhang; Daniel Bennett; Scott E Erdman
Journal:  Eukaryot Cell       Date:  2002-10

4.  Combinatorial regulation of phospholipid biosynthetic gene expression by the UME6, SIN3 and RPD3 genes.

Authors:  M Elkhaimi; M R Kaadige; D Kamath; J C Jackson; H Biliran; J M Lopes
Journal:  Nucleic Acids Res       Date:  2000-08-15       Impact factor: 16.971

5.  Prohibitins act as a membrane-bound chaperone for the stabilization of mitochondrial proteins.

Authors:  L G Nijtmans; L de Jong; M Artal Sanz; P J Coates; J A Berden; J W Back; A O Muijsers; H van der Spek; L A Grivell
Journal:  EMBO J       Date:  2000-06-01       Impact factor: 11.598

6.  The theta subunit of Escherichia coli DNA polymerase III: a role in stabilizing the epsilon proofreading subunit.

Authors:  Sharon A Taft-Benz; Roel M Schaaper
Journal:  J Bacteriol       Date:  2004-05       Impact factor: 3.490

7.  Functional characterization of Pneumocystis carinii brl1 by transspecies complementation analysis.

Authors:  Libera Lo Presti; Moira Cockell; Lorenzo Cerutti; Viesturs Simanis; Philippe M Hauser
Journal:  Eukaryot Cell       Date:  2007-11-09

8.  MluI site-dependent transcriptional regulation of the Candida albicans dUTPase gene.

Authors:  E M McIntosh; J Looser; R H Haynes; R E Pearlman
Journal:  Curr Genet       Date:  1994 Nov-Dec       Impact factor: 3.886

9.  Vps1 in the late endosome-to-vacuole traffic.

Authors:  Jacob Hayden; Michelle Williams; Ann Granich; Hyoeun Ahn; Brandon Tenay; Joshua Lukehart; Chad Highfill; Sarah Dobard; Kyoungtae Kim
Journal:  J Biosci       Date:  2013-03       Impact factor: 1.826

10.  Parameters affecting lithium acetate-mediated transformation of Saccharomyces cerevisiae and development of a rapid and simplified procedure.

Authors:  R Soni; J P Carmichael; J A Murray
Journal:  Curr Genet       Date:  1993-11       Impact factor: 3.886
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