PURPOSE: To investigate the expression and predictive role of the Mre11/Rad50/Nbs1 (MRN) complex and the ataxia-telangiectasia mutated protein (ATM) for the outcome of radiotherapy in breast cancer patients. METHODS AND MATERIALS: The protein expression of ATM and the DNA repair proteins in the MRN complex were investigated using immunohistochemistry in tumors from 224 women with early breast cancer, who were randomized to receive postoperative radiotherapy or adjuvant chemotherapy. RESULTS: Compared with normal breast tissue, the staining intensity of Mre11, Rad50, Nbs1, and ATM was reduced in a majority of the tumors. Weak expression of the MRN complex was correlated with high histologic grade and estrogen receptor negativity (p = 0.01 and p = 0.0001, respectively). Radiotherapy significantly reduced the risk of local recurrence as compared with chemotherapy (p = 0.04). The greatest benefit of radiotherapy was seen in patients with moderate/strong expression of the MRN complex (relative risk = 0.27, 95% confidence interval = 0.098-0.72, p = 0.009), whereas patients with negative/weak MRN expression had no benefit of radiotherapy compared with adjuvant chemotherapy. These results suggest that an intact MRN complex is important for the tumor cell eradicating effect of radiotherapy. CONCLUSIONS: Reduced expression of the MRN complex predicts a poor effect of radiotherapy in patients with early breast cancer.
RCT Entities:
PURPOSE: To investigate the expression and predictive role of the Mre11/Rad50/Nbs1 (MRN) complex and the ataxia-telangiectasia mutated protein (ATM) for the outcome of radiotherapy in breast cancerpatients. METHODS AND MATERIALS: The protein expression of ATM and the DNA repair proteins in the MRN complex were investigated using immunohistochemistry in tumors from 224 women with early breast cancer, who were randomized to receive postoperative radiotherapy or adjuvant chemotherapy. RESULTS: Compared with normal breast tissue, the staining intensity of Mre11, Rad50, Nbs1, and ATM was reduced in a majority of the tumors. Weak expression of the MRN complex was correlated with high histologic grade and estrogen receptor negativity (p = 0.01 and p = 0.0001, respectively). Radiotherapy significantly reduced the risk of local recurrence as compared with chemotherapy (p = 0.04). The greatest benefit of radiotherapy was seen in patients with moderate/strong expression of the MRN complex (relative risk = 0.27, 95% confidence interval = 0.098-0.72, p = 0.009), whereas patients with negative/weak MRN expression had no benefit of radiotherapy compared with adjuvant chemotherapy. These results suggest that an intact MRN complex is important for the tumor cell eradicating effect of radiotherapy. CONCLUSIONS: Reduced expression of the MRN complex predicts a poor effect of radiotherapy in patients with early breast cancer.
Authors: Ananya Choudhury; Louisa D Nelson; Mark T W Teo; Sameer Chilka; Selina Bhattarai; Colin F Johnston; Faye Elliott; Johanna Lowery; Claire F Taylor; Michael Churchman; Johanne Bentley; Margaret A Knowles; Patricia Harnden; Robert G Bristow; D Timothy Bishop; Anne E Kiltie Journal: Cancer Res Date: 2010-09-15 Impact factor: 12.701
Authors: Sylvie M Noordermeer; Marloes Wennemers; Saskia M Bergevoet; Adrian van der Heijden; Evelyn Tönnissen; Fred C G J Sweep; Joop H Jansen; Paul N Span; Bert A van der Reijden Journal: Breast Cancer Res Treat Date: 2012-06-16 Impact factor: 4.872
Authors: Frank Roossink; Hylke W Wieringa; Maartje G Noordhuis; Klaske A ten Hoor; Mirjam Kok; Lorian Slagter-Menkema; Harry Hollema; Geertruida H de Bock; Elisabeth Pras; Elisabeth G E de Vries; Steven de Jong; Ate G J van der Zee; Ed Schuuring; G Bea A Wisman; Marcel A T M van Vugt Journal: Int J Cancer Date: 2012-03-29 Impact factor: 7.396