Literature DB >> 17332412

Reconstitution of DNA segregation driven by assembly of a prokaryotic actin homolog.

Ethan C Garner1, Christopher S Campbell, Douglas B Weibel, R Dyche Mullins.   

Abstract

Multiple unrelated polymer systems have evolved to partition DNA molecules between daughter cells at division. To better understand polymer-driven DNA segregation, we reconstituted the three-component segregation system of the R1 plasmid from purified components. We found that the ParR/parC complex can construct a simple bipolar spindle by binding the ends of ParM filaments, inhibiting dynamic instability, and acting as a ratchet permitting incorporation of new monomers and riding on the elongating filament ends. Under steady-state conditions, the dynamic instability of unattached ParM filaments provides the energy required to drive DNA segregation.

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Year:  2007        PMID： 17332412      PMCID： PMC2851738          DOI： 10.1126/science.1138527

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  15 in total

Review 1.  The polymerization motor.

Authors:  J A Theriot
Journal:  Traffic       Date:  2000-01       Impact factor: 6.215

Review 2.  Plasmid and chromosome partitioning: surprises from phylogeny.

Authors:  K Gerdes; J Møller-Jensen; R Bugge Jensen
Journal:  Mol Microbiol       Date:  2000-08       Impact factor: 3.501

3.  Bacterial DNA segregation by dynamic SopA polymers.

Authors:  Grace E Lim; Alan I Derman; Joe Pogliano
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-23       Impact factor: 11.205

4.  Actin homolog MreB and RNA polymerase interact and are both required for chromosome segregation in Escherichia coli.

Authors:  Thomas Kruse; Blagoy Blagoev; Anders Løbner-Olesen; Masaaki Wachi; Kumi Sasaki; Noritaka Iwai; Matthias Mann; Kenn Gerdes
Journal:  Genes Dev       Date:  2006-01-01       Impact factor: 11.361

5.  F-actin-like filaments formed by plasmid segregation protein ParM.

Authors:  Fusinita van den Ent; Jakob Møller-Jensen; Linda A Amos; Kenn Gerdes; Jan Löwe
Journal:  EMBO J       Date:  2002-12-16       Impact factor: 11.598

6.  Dynamic instability of microtubule growth.

Authors:  T Mitchison; M Kirschner
Journal:  Nature       Date:  1984 Nov 15-21       Impact factor: 49.962

7.  Bacterial mitosis: ParM of plasmid R1 moves plasmid DNA by an actin-like insertional polymerization mechanism.

Authors:  Jakob Møller-Jensen; Jonas Borch; Mette Dam; Rasmus B Jensen; Peter Roepstorff; Kenn Gerdes
Journal:  Mol Cell       Date:  2003-12       Impact factor: 17.970

8.  Prokaryotic DNA segregation by an actin-like filament.

Authors:  Jakob Møller-Jensen; Rasmus Bugge Jensen; Jan Löwe; Kenn Gerdes
Journal:  EMBO J       Date:  2002-06-17       Impact factor: 11.598

9.  Three-dimensional structural characterization of centrosomes from early Drosophila embryos.

Authors:  M Moritz; M B Braunfeld; J C Fung; J W Sedat; B M Alberts; D A Agard
Journal:  J Cell Biol       Date:  1995-09       Impact factor: 10.539

10.  Cell motility by labile association of molecules. The nature of mitotic spindle fibers and their role in chromosome movement.

Authors:  S Inoué; H Sato
Journal:  J Gen Physiol       Date:  1967-07       Impact factor: 4.086
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  113 in total

1.  Structural mechanism of ATP-induced polymerization of the partition factor ParF: implications for DNA segregation.

Authors:  Maria A Schumacher; Qiaozhen Ye; Madhuri T Barge; Massimiliano Zampini; Daniela Barillà; Finbarr Hayes
Journal:  J Biol Chem       Date:  2012-06-06       Impact factor: 5.157

Review 2.  The ParMRC system: molecular mechanisms of plasmid segregation by actin-like filaments.

Authors:  Jeanne Salje; Pananghat Gayathri; Jan Löwe
Journal:  Nat Rev Microbiol       Date:  2010-10       Impact factor: 60.633

Review 3.  The structure and function of bacterial actin homologs.

Authors:  Joshua W Shaevitz; Zemer Gitai
Journal:  Cold Spring Harb Perspect Biol       Date:  2010-07-14       Impact factor: 10.005

Review 4.  Cell mechanics and the cytoskeleton.

Authors:  Daniel A Fletcher; R Dyche Mullins
Journal:  Nature       Date:  2010-01-28       Impact factor: 49.962

Review 5.  Reaction-diffusion systems in intracellular molecular transport and control.

Authors:  Siowling Soh; Marta Byrska; Kristiana Kandere-Grzybowska; Bartosz A Grzybowski
Journal:  Angew Chem Int Ed Engl       Date:  2010-06-07       Impact factor: 15.336

6.  Independent segregation of the two arms of the Escherichia coli ori region requires neither RNA synthesis nor MreB dynamics.

Authors:  Xindan Wang; David J Sherratt
Journal:  J Bacteriol       Date:  2010-10-01       Impact factor: 3.490

7.  The tubulin-like RepX protein encoded by the pXO1 plasmid forms polymers in vivo in Bacillus anthracis.

Authors:  Parvez Akhtar; Syam P Anand; Simon C Watkins; Saleem A Khan
Journal:  J Bacteriol       Date:  2009-02-20       Impact factor: 3.490

Review 8.  The biology of boundary conditions: cellular reconstitution in one, two, and three dimensions.

Authors:  Michael D Vahey; Daniel A Fletcher
Journal:  Curr Opin Cell Biol       Date:  2013-11-12       Impact factor: 8.382

9.  A prophage-encoded actin-like protein required for efficient viral DNA replication in bacteria.

Authors:  Catriona Donovan; Antonia Heyer; Eugen Pfeifer; Tino Polen; Anja Wittmann; Reinhard Krämer; Julia Frunzke; Marc Bramkamp
Journal:  Nucleic Acids Res       Date:  2015-04-27       Impact factor: 16.971

10.  Kinetochores and microtubules wed without a ring.

Authors:  Kerry Bloom
Journal:  Cell       Date:  2008-10-17       Impact factor: 41.582
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