OBJECTIVE: To examine the utility of in vivo elastic light scattering measurements to diagnose high grade squamous interepithelial lesions (HSIL) of the cervix. METHODS: A newly developed fiber optic probe was used to measure light transport in the cervical epithelium of 36 patients undergoing standard colposcopy. Both unpolarized and polarized light transport were measured in the visible and near-infrared. Spectroscopic results of 29 patients were compared with histopathology of the measured sites using ROC curves, MANOVA and logistic regression. RESULTS: Three spectroscopic parameters are statistically different for HSIL compared with low-grade lesions and normal tissue. When these three spectroscopic parameters are combined, retrospective sensitivities and specificities for HSIL versus non-HSIL are 100% and 80%, respectively. CONCLUSIONS: Reflectance measurements of elastically scattered light show promise as a non-invasive, real-time method to discriminate HSIL from other abnormalities and normal tissue. These results compare favorably with those obtained by fluorescence alone and by fluorescence combined with light scattering.
OBJECTIVE: To examine the utility of in vivo elastic light scattering measurements to diagnose high grade squamous interepithelial lesions (HSIL) of the cervix. METHODS: A newly developed fiber optic probe was used to measure light transport in the cervical epithelium of 36 patients undergoing standard colposcopy. Both unpolarized and polarized light transport were measured in the visible and near-infrared. Spectroscopic results of 29 patients were compared with histopathology of the measured sites using ROC curves, MANOVA and logistic regression. RESULTS: Three spectroscopic parameters are statistically different for HSIL compared with low-grade lesions and normal tissue. When these three spectroscopic parameters are combined, retrospective sensitivities and specificities for HSIL versus non-HSIL are 100% and 80%, respectively. CONCLUSIONS: Reflectance measurements of elastically scattered light show promise as a non-invasive, real-time method to discriminate HSIL from other abnormalities and normal tissue. These results compare favorably with those obtained by fluorescence alone and by fluorescence combined with light scattering.
Authors: Vivide Tuan-Chyan Chang; Peter S Cartwright; Sarah M Bean; Greg M Palmer; Rex C Bentley; Nirmala Ramanujam Journal: Neoplasia Date: 2009-04 Impact factor: 5.715
Authors: Condon Lau; Jelena Mirkovic; Chung-Chieh Yu; Geoff P O'Donoghue; Luis Galindo; Ramachandra Dasari; Antonio de las Morenas; Michael Feld; Elizabeth Stier Journal: J Biomed Opt Date: 2013-07 Impact factor: 3.170
Authors: Pilar Beatriz Garcia-Allende; Venkataramanan Krishnaswamy; P Jack Hoopes; Kimberley S Samkoe; Olga M Conde; Brian W Pogue Journal: J Biomed Opt Date: 2009 May-Jun Impact factor: 3.170
Authors: Scott B Cantor; Jose-Miguel Yamal; Martial Guillaud; Dennis D Cox; E Neely Atkinson; John L Benedet; Dianne Miller; Thomas Ehlen; Jasenka Matisic; Dirk van Niekerk; Monique Bertrand; Andrea Milbourne; Helen Rhodes; Anais Malpica; Gregg Staerkel; Shahla Nader-Eftekhari; Karen Adler-Storthz; Michael E Scheurer; Karen Basen-Engquist; Eileen Shinn; Loyd A West; Anne-Therese Vlastos; Xia Tao; J Robert Beck; Calum Macaulay; Michele Follen Journal: Int J Cancer Date: 2010-11-09 Impact factor: 7.396
Authors: Jose-Miguel Yamal; Getie A Zewdie; Dennis D Cox; E Neely Atkinson; Scott B Cantor; Calum MacAulay; Kalatu Davies; Isaac Adewole; Timon P H Buys; Michele Follen Journal: J Biomed Opt Date: 2012-04 Impact factor: 3.170
Authors: Judith R Mourant; Tamara M Powers; Thérese J Bocklage; Heather M Greene; Maxine H Dorin; Alan G Waxman; Meggan M Zsemlye; Harriet O Smith Journal: Appl Opt Date: 2009-04-01 Impact factor: 1.980