Literature DB >> 1729687

Yeast artificial chromosomes spanning 8 megabases and 10-15 centimorgans of human cytogenetic band Xq26.

R D Little1, G Pilia, S Johnson, M D'Urso, D Schlessinger.   

Abstract

A successful test is reported to generate long-range contiguous coverage of DNA from a human cytogenetic band in overlapping yeast artificial chromosomes (YACs). Seed YACs in band Xq26 were recovered from a targeted library of clones from Xq24-q28 with 14 probes, including probes for the hypoxanthine guanine phosphoribosyltransferase- and coagulation factor IX-encoding genes and nine probes used in linkage mapping. Neighboring YACs were then identified by 25 "walking" steps with end-clones, and the content of 71 probes in cognate YACs was verified by further hybridization analyses. The resultant contig extends across 8 million base pairs, including most of band Xq26, with an order of markers consistent with linkage data. YAC-based mapping, thus, permits steps toward a fully integrated physical and genetic map and is probably adequate to sustain most of the human genome project.

Entities:  

Mesh：

Substances：

Year:  1992        PMID： 1729687      PMCID： PMC48199          DOI： 10.1073/pnas.89.1.177

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  27 in total

1.  The human HPRT gene on a yeast artificial chromosome is functional when transferred to mouse cells by cell fusion.

Authors:  C Huxley; Y Hagino; D Schlessinger; M V Olson
Journal:  Genomics       Date:  1991-04       Impact factor: 5.736

2.  In situ hybridization to cytogenetic bands of yeast artificial chromosomes covering 50% of human Xq24-Xq28 DNA.

Authors:  V Montanaro; A Casamassimi; M D'Urso; J Y Yoon; W Freije; D Schlessinger; M Muenke; R L Nussbaum; S Saccone; S Maugeri
Journal:  Am J Hum Genet       Date:  1991-02       Impact factor: 11.025

3.  Wilms tumor locus on 11p13 defined by multiple CpG island-associated transcripts.

Authors:  L Bonetta; S E Kuehn; A Huang; D J Law; L M Kalikin; M Koi; A E Reeve; B H Brownstein; H Yeger; B R Williams
Journal:  Science       Date:  1990-11-16       Impact factor: 47.728

4.  Automated DNA sequencing of the human HPRT locus.

Authors:  A Edwards; H Voss; P Rice; A Civitello; J Stegemann; C Schwager; J Zimmermann; H Erfle; C T Caskey; W Ansorge
Journal:  Genomics       Date:  1990-04       Impact factor: 5.736

5.  Yeast artificial chromosome cloning of a two-megabase-size contig within chromosomal band 18q21 establishes physical linkage between BCL2 and plasminogen activator inhibitor type-2.

Authors:  G A Silverman; J I Jockel; P H Domer; R M Mohr; P Taillon-Miller; S J Korsmeyer
Journal:  Genomics       Date:  1991-02       Impact factor: 5.736

6.  Stable integration and expression in mouse cells of yeast artificial chromosomes harboring human genes.

Authors:  B Eliceiri; T Labella; Y Hagino; A Srivastava; D Schlessinger; G Pilia; G Palmieri; M D'Urso
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

Review 7.  Yeast artificial chromosomes: tools for mapping and analysis of complex genomes.

Authors:  D Schlessinger
Journal:  Trends Genet       Date:  1990-08       Impact factor: 11.639

8.  Yeast artificial chromosomes containing human Xq24-Xq28 DNA: library construction and representation of probe sequences.

Authors:  F E Abidi; M Wada; R D Little; D Schlessinger
Journal:  Genomics       Date:  1990-07       Impact factor: 5.736

9.  Human Xq24-Xq28: approaches to mapping with yeast artificial chromosomes.

Authors:  M Wada; R D Little; F Abidi; G Porta; T Labella; T Cooper; G Della Valle; M D'Urso; D Schlessinger
Journal:  Am J Hum Genet       Date:  1990-01       Impact factor: 11.025

Review 10.  Construction of a genetic linkage map in man using restriction fragment length polymorphisms.

Authors:  D Botstein; R L White; M Skolnick; R W Davis
Journal:  Am J Hum Genet       Date:  1980-05       Impact factor: 11.025
View more
  14 in total

1.  YAC clone contigs covering 5 Mb of a repeat sequence island on the mouse X chromosome.

Authors:  P Mileham; S D Brown
Journal:  Mamm Genome       Date:  1996-04       Impact factor: 2.957

2.  Chimeric YACs were generated at unreduced rates in conditions that suppress coligation.

Authors:  M Wada; K Abe; K Okumura; H Taguchi; K Kohno; F Imamoto; D Schlessinger; M Kuwano
Journal:  Nucleic Acids Res       Date:  1994-05-11       Impact factor: 16.971

3.  Linkage mapping of a severe X-linked mental retardation syndrome.

Authors:  H Malmgren; M Sundvall; N Dahl; K H Gustavson; G Annerén; C Wadelius; M L Steén-Bondeson; U Pettersson
Journal:  Am J Hum Genet       Date:  1993-06       Impact factor: 11.025

4.  Rapid and efficient construction of yeast artificial chromosome contigs in the mouse genome with interspersed repetitive sequence PCR (IRS-PCR): generation of a 5-cM, > 5 megabase contig on mouse chromosome 1.

Authors:  K W Hunter; S D Ontiveros; M L Watson; V P Stanton; P Gutierrez; D Bhat; J Rochelle; S Graw; C Ton; M Schalling
Journal:  Mamm Genome       Date:  1994-10       Impact factor: 2.957

5.  Molecular genetic haplotype segregation studies in three families with X-linked lymphoproliferative disease.

Authors:  V Schuster; S Seidenspinner; T Grimm; W Kress; S Zielen; M Bock; H W Kreth
Journal:  Eur J Pediatr       Date:  1994-06       Impact factor: 3.183

6.  Mapping of the X linked form of hyper IgM syndrome (HIGM1)

Authors:  M Padayachee; R J Levinsky; C Kinnon; A Finn; C McKeown; C Feighery; L D Notarangelo; R W Hendriks; A P Read; S Malcolm
Journal:  J Med Genet       Date:  1993-03       Impact factor: 6.318

7.  Characterization of four human YAC libraries for clone size, chimerism and X chromosome sequence representation.

Authors:  R Nagaraja; J Kere; S MacMillan; M J Masisi; D Johnson; B J Molini; G R Halley; K Wein; M Trusgnich; B Eble
Journal:  Nucleic Acids Res       Date:  1994-08-25       Impact factor: 16.971

8.  Introduction of YACs into intact yeast cells by a procedure which shows low levels of recombinagenicity and co-transformation.

Authors:  S M Heale; L I Stateva; S G Oliver
Journal:  Nucleic Acids Res       Date:  1994-11-25       Impact factor: 16.971

9.  An ordered collection of Bacillus subtilis DNA segments cloned in yeast artificial chromosomes.

Authors:  V Azevedo; E Alvarez; E Zumstein; G Damiani; V Sgaramella; S D Ehrlich; P Serror
Journal:  Proc Natl Acad Sci U S A       Date:  1993-07-01       Impact factor: 11.205

10.  Chromosome walking with YAC clones in Arabidopsis: isolation of 1700 kb of contiguous DNA on chromosome 5, including a 300 kb region containing the flowering-time gene CO.

Authors:  J Putterill; F Robson; K Lee; G Coupland
Journal:  Mol Gen Genet       Date:  1993-05
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.