OBJECTIVES: We aimed to establish the earliest gestational age at which fetal DNA in maternal plasma could be detected and whether this was reliable at 12-13 weeks' gestation. STUDY DESIGN: A prospective observational cohort study of 32 pregnancies either after IVF or before prenatal diagnosis by CVS. Maternal blood was taken and RT-PCR was carried out to detect the multi-copy Y chromosome associated DSY14 gene. The end point was gender as assessed at delivery or on karyotype. RESULTS: Y signal was obtained as early as 14 days post conception (4 weeks' gestation) and has a good prediction rate by 12 weeks' gestation. CONCLUSION: Free fetal DNA allows very early prediction of fetal sex in some cases and could be useful for clinical use for X-linked conditions by the end of the first trimester.
OBJECTIVES: We aimed to establish the earliest gestational age at which fetal DNA in maternal plasma could be detected and whether this was reliable at 12-13 weeks' gestation. STUDY DESIGN: A prospective observational cohort study of 32 pregnancies either after IVF or before prenatal diagnosis by CVS. Maternal blood was taken and RT-PCR was carried out to detect the multi-copy Y chromosome associated DSY14 gene. The end point was gender as assessed at delivery or on karyotype. RESULTS: Y signal was obtained as early as 14 days post conception (4 weeks' gestation) and has a good prediction rate by 12 weeks' gestation. CONCLUSION: Free fetal DNA allows very early prediction of fetal sex in some cases and could be useful for clinical use for X-linked conditions by the end of the first trimester.
Authors: Antina de Jong; Wybo J Dondorp; Christine E M de Die-Smulders; Suzanne G M Frints; Guido M W R de Wert Journal: Eur J Hum Genet Date: 2009-12-02 Impact factor: 4.246
Authors: Ji Hyae Lim; Bom Yi Lee; Jin Woo Kim; You Jung Han; Jin Hoon Chung; Min Hyoung Kim; Dong Wook Kwak; So Yeon Park; Hee Back Choi; Hyun Mee Ryu Journal: J Assist Reprod Genet Date: 2018-02-08 Impact factor: 3.412
Authors: Hada C Macher; Maria A Martinez-Broca; Amalia Rubio-Calvo; Cristina Leon-Garcia; Manuel Conde-Sanchez; Alzenira Costa; Elena Navarro; Juan M Guerrero Journal: PLoS One Date: 2012-12-07 Impact factor: 3.240