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Literature DB >> 17215870

Investigating membrane protein dynamics in living cells.

Ian R Bates¹, Paul W Wiseman, John W Hanrahan.

Abstract

Live cell imaging is a powerful tool for understanding the function and regulation of membrane proteins. In this review, we briefly discuss 4 fluorescence-microscopy-based techniques for studying the transport dynamics of membrane proteins: fluorescence-correlation spectroscopy, image-correlation spectroscopy, fluorescence recovery after photobleaching, and single-particle and (or) molecule tracking. The advantages and limitations of each approach are illustrated using recent studies of an ion channel and cell adhesion molecules.

Mesh：

Substances：
Membrane Proteins

Year: 2006 PMID： 17215870 DOI： 10.1139/o06-189

Source DB: PubMed Journal: Biochem Cell Biol ISSN： 0829-8211 Impact factor: 3.626

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Cited

15 in total

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Journal: Nat Methods Date: 2007-08-05 Impact factor: 28.547

Review 2. New gateways to discovery.

Authors: Michael M Goodin; Romit Chakrabarty; Rituparna Banerjee; Sharon Yelton; Seth Debolt
Journal: Plant Physiol Date: 2007-12 Impact factor: 8.340

Review 3. Functional membrane diffusion of G-protein coupled receptors.

Authors: Aurélie Baker; Aude Saulière; Fabrice Dumas; Claire Millot; Serge Mazères; André Lopez; Laurence Salomé
Journal: Eur Biophys J Date: 2007-09-26 Impact factor: 1.733

4. Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy.

Authors: Ariel Michelman-Ribeiro; Davide Mazza; Tilman Rosales; Timothy J Stasevich; Hacene Boukari; Vikas Rishi; Charles Vinson; Jay R Knutson; James G McNally
Journal: Biophys J Date: 2009-07-08 Impact factor: 4.033

5. Going beyond 2D: Following membrane diffusion and topography in the IgE-Fc[Epsilon]RI system using 3-dimensional tracking microscopy.

Authors: Nathan P Wells; Guillaume A Lessard; Mary E Phipps; Peter M Goodwin; Diane S Lidke; Bridget S Wilson; James H Werner
Journal: Proc SPIE Int Soc Opt Eng Date: 2009-02-24

6. Educated natural killer cells show dynamic movement of the activating receptor NKp46 and confinement of the inhibitory receptor Ly49A.

Authors: Elina Staaf; Per Niklas Hedde; Sunitha Bagawath Singh; Joachim Piguet; Enrico Gratton; Sofia Johansson
Journal: Sci Signal Date: 2018-02-13 Impact factor: 8.192

Investigating membrane protein dynamics in living cells.

1. An HSV vector system for selection of ligand-gated ion channel modulators.

Review 2. New gateways to discovery.

Review 3. Functional membrane diffusion of G-protein coupled receptors.

4. Direct measurement of association and dissociation rates of DNA binding in live cells by fluorescence correlation spectroscopy.

5. Going beyond 2D: Following membrane diffusion and topography in the IgE-Fc[Epsilon]RI system using 3-dimensional tracking microscopy.

6. Educated natural killer cells show dynamic movement of the activating receptor NKp46 and confinement of the inhibitory receptor Ly49A.

7. Detection of membrane-bound and soluble antigens by magnetic levitation.

8. Image correlation microscopy for uniform illumination.

9. Dynamic imaging of molecular assemblies in live cells based on nanoparticle plasmon resonance coupling.

10. Cholesterol modulation of nicotinic acetylcholine receptor surface mobility.