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Literature DB >> 17177349

The in vitro characterization of the iterative avermectin glycosyltransferase AveBI reveals reaction reversibility and sugar nucleotide flexibility.

Changsheng Zhang¹, Christoph Albermann, Xun Fu, Jon S Thorson.

Abstract

The glycosyltransferase AveBI, which is involved in the biosynthesis of the macrolide antihelmintic avermectin (AVM), was characterized in vitro. AveBI was confirmed to catalyze two separate iterative additions of l-oleandrose, and the reversibility of AveBI-catalyzed reaction was also demonstrated. Investigation of sugar nucleotide specificity revealed 10 unique sugar nucleotide substrates which, in combination with five distinct aglycones, led to the production of 50 differentially glycosylated AVM variants.

Entities: Chemical

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Year: 2006 PMID： 17177349 DOI： 10.1021/ja065950k

Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419

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Cited

24 in total

1. Preliminary X-ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species.

Authors: Liping Gong; Yi Xiao; Qiang Liu; Sumei Li; Changsheng Zhang; Jinsong Liu
Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun Date: 2010-12-23

2. Probing the aglycon promiscuity of an engineered glycosyltransferase.

Authors: Richard W Gantt; Randal D Goff; Gavin J Williams; Jon S Thorson
Journal: Angew Chem Int Ed Engl Date: 2008 Impact factor: 15.336

3. Tracking down biotransformation to the genetic level: identification of a highly flexible glycosyltransferase from Saccharothrix espanaensis.

Authors: Tina Strobel; Yvonne Schmidt; Anton Linnenbrink; Andriy Luzhetskyy; Marta Luzhetska; Takaaki Taguchi; Elke Brötz; Thomas Paululat; Maryna Stasevych; Oleg Stanko; Volodymyr Novikov; Andreas Bechthold
Journal: Appl Environ Microbiol Date: 2013-06-21 Impact factor: 4.792

4. The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI.

Authors: Changsheng Zhang; Rocco Moretti; Jiqing Jiang; Jon S Thorson
Journal: Chembiochem Date: 2008-10-13 Impact factor: 3.164

5. Optimizing glycosyltransferase specificity via "hot spot" saturation mutagenesis presents a catalyst for novobiocin glycorandomization.

Authors: Gavin J Williams; Randal D Goff; Changsheng Zhang; Jon S Thorson
Journal: Chem Biol Date: 2008-04

The in vitro characterization of the iterative avermectin glycosyltransferase AveBI reveals reaction reversibility and sugar nucleotide flexibility.

1. Preliminary X-ray crystallographic analysis of the glycosyltransferase from a marine Streptomyces species.

2. Probing the aglycon promiscuity of an engineered glycosyltransferase.

3. Tracking down biotransformation to the genetic level: identification of a highly flexible glycosyltransferase from Saccharothrix espanaensis.

4. The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI.

5. Optimizing glycosyltransferase specificity via "hot spot" saturation mutagenesis presents a catalyst for novobiocin glycorandomization.

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