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Literature DB >> 18798210

The in vitro characterization of polyene glycosyltransferases AmphDI and NysDI.

Changsheng Zhang¹, Rocco Moretti, Jiqing Jiang, Jon S Thorson.

Abstract

The overproduction, purification, and in vitro characterization of the polyene glycosyltransferases (GTs) AmphDI and NysDI are reported. A novel nucleotidyltransferase mutant (RmlA Q83D) for the chemoenzymatic synthesis of unnatural GDP-sugar donors in conjunction with polyene GT-catalyzed sugar exchange/reverse reactions allowed the donor and acceptor specificities of these novel enzymes to be probed. The evaluation of polyene GT aglycon and GDP-sugar donor specificity revealed some tolerance to aglycon structural diversity, but stringent sugar specificity, and culminated in new polyene analogues in which L-gulose or D-mannose replace the native sugar D-mycosamine.

Entities: CellLine Chemical Disease Gene Mutation Species

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Year: 2008 PMID： 18798210 PMCID： PMC2947747 DOI： 10.1002/cbic.200800349

Source DB: PubMed Journal: Chembiochem ISSN： 1439-4227 Impact factor: 3.164

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