Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Recombinant single chain Fv antibodies specific for glycoprotein D of equid herpesvirus 1.

Literature DB >> 17176773

Recombinant single chain Fv antibodies specific for glycoprotein D of equid herpesvirus 1.

Abstract

Single chain Fv (scFv) molecules generated by phage-display technology represent a new and efficient tool in the research and diagnostics of infectious diseases. The recombinant glycoprotein D of Equid herpesvirus 1 was successfully expressed in E. coli cells. The protein was produced predominantly in soluble fraction and was then purified on a nickel-agarose column. The scFv antibodies against the glycoprotein were selected and several clones of glycoprotein D-specific scFv-antibodies were identified; t of them was expressed as a soluble scFv molecule, purified by immobilized metal-affinity chromatography and used as reagent in an immunofluorescence test.

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 2006 PMID： 17176773 DOI： 10.1007/bf02931597

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

25 in total

1. Recombinant single-chain Fv antibodies that recognize the p25 protein of the Maedi-Visna virus.

Authors: V Celer; D Blazek; I Navrátilová; P Skládal; B Blacklaws; R Bujdoso
Journal: Folia Microbiol (Praha) Date: 2003 Impact factor: 2.099

2. An outbreak of Equid herpesvirus abortion in New South Wales.

Authors: M Carrigan; P Cosgrove; P Kirkland; M Sabine
Journal: Equine Vet J Date: 1991-03 Impact factor: 2.888

3. Single-chain variable fragments selected on the 57-76 p21Ras neutralising epitope from phage antibody libraries recognise the parental protein.

Authors: L Persic; I R Horn; S Rybak; A Cattaneo; H R Hoogenboom; A Bradbury
Journal: FEBS Lett Date: 1999-01-25 Impact factor: 4.124

4. Model systems to study the parameters determining the success of phage antibody selections on complex antigens.

Authors: R Mutuberria; H R Hoogenboom; E van der Linden; A P de Bruïne; R C Roovers
Journal: J Immunol Methods Date: 1999-12-10 Impact factor: 2.303

5. Phage-displayed and soluble mouse scFv fragments neutralize rabies virus.

Authors: B H Muller; F Lafay; C Demangel; P Perrin; N Tordo; A Flamand; P Lafaye; J L Guesdon
Journal: J Virol Methods Date: 1997-09 Impact factor: 2.014

6. Cloning, expression and functional activities of a single chain antibody fragment directed to fusion protein of respiratory syncytial virus.

Authors: F Guirakhoo; J Catalan; T Monath; R Weltzin
Journal: Immunotechnology Date: 1996-09

7. Equine herpesvirus 1 glycoprotein D: mapping of the transcript and a neutralization epitope.

Authors: C C Flowers; D J O'Callaghan
Journal: J Virol Date: 1992-11 Impact factor: 5.103

8. Major histocompatibility complex class I-restricted cytotoxic T-lymphocyte responses in horses infected with equine herpesvirus 1.

Authors: G Allen; M Yeargan; L R Costa; R Cross
Journal: J Virol Date: 1995-01 Impact factor: 5.103