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Literature DB >> 19381477

The selection of single-chain Fv antibody fragments specific to Bhlp 29.7 protein of Brachyspira hyodysenteriae.

Abstract

Single-chain antibodies (scFv) specific to Brachyspira hyodysenteriae were isolated from a phagemid library. Recombinant Bhlp 29.7 protein was used for scFv selection and individual clones were tested by ELISA and immunofluorescent test; four unique clones were isolated. One of selected clones was able to bind specifically B. hyodysenteriae in ELISA and immunofluorescence test. This is the first report of species-specific recombinant antibodies against B. hyodysenteriae.

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Year: 2009 PMID： 19381477 DOI： 10.1007/s12223-008-0081-3

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

12 in total

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Journal: Vet Microbiol Date: 2005-05-20 Impact factor: 3.293

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Journal: J Immunol Methods Date: 2006-04-21 Impact factor: 2.303

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Authors: Si Wu; Ailong Ke; Jennifer A Doudna
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Journal: Anim Health Res Rev Date: 2001-06 Impact factor: 2.615

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9. Production and characterization of monoclonal antibodies against Serpulina hyodysenteriae and S. innocens and their use in serotyping.

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