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Literature DB >> 12879760

Recombinant single-chain Fv antibodies that recognize the p25 protein of the Maedi-Visna virus.

V Celer¹, D Blazek, I Navrátilová, P Skládal, B Blacklaws, R Bujdoso.

Abstract

Single chain Fv (scFv) antibodies (generated by phage display technology, molecules representing new and efficient tools in the research and diagnostics of infectious diseases) against the capsid protein (p25) of Maedi-Visna virus were selected. Several clones of p25 specific scFv antibodies were identified; one of them was expressed as a soluble scFv molecule, purified by immobilized metal-affinity chromatography and further characterized by sequencing and determination of the kinetic equilibrium association constant. Sequence analysis showed that the rearranged VL and VH domains of the analyzed scFv clone used sequences from the VL3 family (germline DPL16/VL3.1) and VH1 family (germline VH20), respectively. The kinetic equilibrium association constant was determined as KA = 1.12 +/- 0.52 L/mumol.

Entities: Chemical Disease Gene Species

Mesh：

Substances：

Year: 2003 PMID： 12879760 DOI： 10.1007/bf02931380

Source DB: PubMed Journal: Folia Microbiol (Praha) ISSN： 0015-5632 Impact factor: 2.099

21 in total

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Journal: Cell Date: 1985-08 Impact factor: 41.582

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Journal: Biophys J Date: 1996-03 Impact factor: 4.033

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Authors: H E Gendelman; O Narayan; S Kennedy-Stoskopf; P G Kennedy; Z Ghotbi; J E Clements; J Stanley; G Pezeshkpour
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Review 4. Generating improved single-chain Fv molecules for tumor targeting.

Authors: G P Adams; R Schier
Journal: J Immunol Methods Date: 1999-12-10 Impact factor: 2.303

5. Phage-displayed and soluble mouse scFv fragments neutralize rabies virus.

Authors: B H Muller; F Lafay; C Demangel; P Perrin; N Tordo; A Flamand; P Lafaye; J L Guesdon
Journal: J Virol Methods Date: 1997-09 Impact factor: 2.014

6. Neutralizing human anti crotoxin scFv isolated from a nonimmunized phage library.

Authors: D F Cardoso; F Nato; P England; M L Ferreira; T J Vaughan; I Mota; J C Mazie; V Choumet; P Lafaye
Journal: Scand J Immunol Date: 2000-04 Impact factor: 3.487

Review 7. Visna-maedi in sheep.

Authors: M Gudnadóttir
Journal: Prog Med Virol Date: 1974

Review 8. Ras and p53 intracellular targeting with recombinant single-chain Fv (scFv) fragments: a novel approach for cancer therapy?

Authors: O Cochet; N Gruel; W H Fridman; J L Teillaud
Journal: Cancer Detect Prev Date: 1999

Recombinant single-chain Fv antibodies that recognize the p25 protein of the Maedi-Visna virus.

1. Nucleotide sequence of the visna lentivirus: relationship to the AIDS virus.

2. Kinetics of ligand binding to receptor immobilized in a polymer matrix, as detected with an evanescent wave biosensor. I. A computer simulation of the influence of mass transport.

3. Tropism of sheep lentiviruses for monocytes: susceptibility to infection and virus gene expression increase during maturation of monocytes to macrophages.

Review 4. Generating improved single-chain Fv molecules for tumor targeting.

5. Phage-displayed and soluble mouse scFv fragments neutralize rabies virus.

6. Neutralizing human anti crotoxin scFv isolated from a nonimmunized phage library.

Review 7. Visna-maedi in sheep.

Review 8. Ras and p53 intracellular targeting with recombinant single-chain Fv (scFv) fragments: a novel approach for cancer therapy?

9. Development of a monoclonal antibody blocking-ELISA for detection of antibodies against Maedi-Visna virus.

10. By-passing immunization. Human antibodies from V-gene libraries displayed on phage.

Review 1. The production and application of single-chain antibody fragments.

2. Recombinant single chain Fv antibodies specific for glycoprotein D of equid herpesvirus 1.