Literature DB >> 1716297

Detection of human immunodeficiency virus type 1 genomic RNA in plasma samples by reverse-transcription polymerase chain reaction.

P Bagnarelli1, S Menzo, A Manzin, M Giacca, P E Varaldo, M Clementi.   

Abstract

An application of the polymerase chain reaction (PCR) to the direct detection of human immunodeficiency virus type 1 (HIV-1) viremia is described. The amplification of specific HIV-1 sequences of gag and env viral genes was carried out after the reverse-transcription of plasma samples (plasma RT-PCR) from seropositive subjects. The assay is faster and cheaper than detection of specific HIV-1 transcripts from peripheral blood mononuclear cells by RT-PCR. The data suggest that HIV-1 viremia is detectable by plasma RT-PCR in a large proportion of seropositive individuals.

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Year:  1991        PMID： 1716297     DOI： 10.1002/jmv.1890340204

Source DB:  PubMed          Journal:  J Med Virol        ISSN： 0146-6615            Impact factor:   2.327


  16 in total

1.  Absolute quantitation of viremia in human immunodeficiency virus infection by competitive reverse transcription and polymerase chain reaction.

Authors:  S Menzo; P Bagnarelli; M Giacca; A Manzin; P E Varaldo; M Clementi
Journal:  J Clin Microbiol       Date:  1992-07       Impact factor: 5.948

Review 2.  Clinical use of quantitative molecular methods in studying human immunodeficiency virus type 1 infection.

Authors:  M Clementi; S Menzo; P Bagnarelli; A Valenza; S Paolucci; R Sampaolesi; A Manzin; P E Varaldo
Journal:  Clin Microbiol Rev       Date:  1996-04       Impact factor: 26.132

Review 3.  Update on microbicide research and development - seeking new HIV prevention tools for women.

Authors:  T Mertenskoetter; Paulina E Kaptur
Journal:  Eur J Med Res       Date:  2011-01-27       Impact factor: 2.175

4.  Dynamics and modulation of human immunodeficiency virus type 1 transcripts in vitro and in vivo.

Authors:  P Bagnarelli; A Valenza; S Menzo; R Sampaolesi; P E Varaldo; L Butini; M Montroni; C F Perno; S Aquaro; D Mathez; J Leibowitch; C Balotta; M Clementi
Journal:  J Virol       Date:  1996-11       Impact factor: 5.103

Review 5.  Quantitative molecular methods in virology.

Authors:  M Clementi; S Menzo; A Manzin; P Bagnarelli
Journal:  Arch Virol       Date:  1995       Impact factor: 2.574

6.  Rapid and simple PCR assay for quantitation of human immunodeficiency virus type 1 RNA in plasma: application to acute retroviral infection.

Authors:  J Mulder; N McKinney; C Christopherson; J Sninsky; L Greenfield; S Kwok
Journal:  J Clin Microbiol       Date:  1994-02       Impact factor: 5.948

7.  The U3 promoter region of the acutely lethal simian immunodeficiency virus clone smmPBj1.9 confers related biological activity on the apathogenic clone agm3mc.

Authors:  M T Dittmar; K Cichutek; P N Fultz; R Kurth
Journal:  Proc Natl Acad Sci U S A       Date:  1995-02-28       Impact factor: 11.205

8.  Anti-V3 loop spectrotype in HIV-infected individuals during zidovudine therapy.

Authors:  R Biselli; V Del Bono; M Anselmo; A Canessa; G Mazzarello; R D'Amelio; D Bassetti
Journal:  Infection       Date:  1996 May-Jun       Impact factor: 3.553

9.  Human immunodeficiency virus type 1 and hepatitis B virus transcription in peripheral blood lymphocytes from co-infected subjects.

Authors:  M Clementi; A Manzin; P Bagnarelli; S Menzo; P E Varaldo; G Carloni
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

10.  Quantitative molecular monitoring of human immunodeficiency virus type 1 activity during therapy with specific antiretroviral compounds.

Authors:  P Bagnarelli; S Menzo; A Valenza; S Paolucci; S Petroni; G Scalise; R Sampaolesi; A Manzin; P E Varaldo; M Clementi
Journal:  J Clin Microbiol       Date:  1995-01       Impact factor: 5.948
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