OBJECTIVES: This study was performed to test a new technique for treatment of chronic non-healing wound (diabetic ulcer) using autologous biograft composed of autologous skin fibroblasts on biodegradable collagen membrane (Coladerm) in combination with autologous mesenchymal stem cells (MSC) derived from the patient's bone marrow. DESIGN: The bone marrow aspirate of the patient with diabetic foot was applied directly to the wound and injected into the edges of the wound, finally covered with prepared autologous biograft. The patient received two additional treatments with cultured MSC on day 7 and 17. RESULTS: The wound showed a steady overall decrease in wound size and an increase in the vascularity of the dermis and in the dermal thickness of the wound bed after 29 days of combined treatment. CONCLUSIONS: Closing and healing of the non-healing diabetic ulcer was achieved by using the given combined therapy.
OBJECTIVES: This study was performed to test a new technique for treatment of chronic non-healing wound (diabetic ulcer) using autologous biograft composed of autologous skin fibroblasts on biodegradable collagen membrane (Coladerm) in combination with autologous mesenchymal stem cells (MSC) derived from the patient's bone marrow. DESIGN: The bone marrow aspirate of the patient with diabetic foot was applied directly to the wound and injected into the edges of the wound, finally covered with prepared autologous biograft. The patient received two additional treatments with cultured MSC on day 7 and 17. RESULTS: The wound showed a steady overall decrease in wound size and an increase in the vascularity of the dermis and in the dermal thickness of the wound bed after 29 days of combined treatment. CONCLUSIONS: Closing and healing of the non-healing diabetic ulcer was achieved by using the given combined therapy.
Authors: Qing Lin; Russell N Wesson; Hiromichi Maeda; Yongchun Wang; Zhu Cui; Jun O Liu; Andrew M Cameron; Bin Gao; Robert A Montgomery; George M Williams; Zhaoli Sun Journal: J Invest Dermatol Date: 2014-03-28 Impact factor: 8.551
Authors: Christine Linard; Frederique Tissedre; Elodie Busson; Valerie Holler; Thomas Leclerc; Carine Strup-Perrot; Ludovic Couty; Bruno L'homme; Marc Benderitter; Antoine Lafont; Jean Jacques Lataillade; Bernard Coulomb Journal: Stem Cells Dev Date: 2015-02-26 Impact factor: 3.272
Authors: Scott Maxson; Erasmo A Lopez; Dana Yoo; Alla Danilkovitch-Miagkova; Michelle A Leroux Journal: Stem Cells Transl Med Date: 2012-02 Impact factor: 6.940
Authors: Bruno Amato; Rita Compagna; Maurizio Amato; Lucia Butrico; Francesco Fugetto; Mariia D Chibireva; Andrea Barbetta; Marco Cannistrà; Stefano de Franciscis; Raffaele Serra Journal: Int Wound J Date: 2015-09-24 Impact factor: 3.315