Literature DB >> 17142898

Crystallization and X-ray diffraction analysis of 6-aminohexanoate-cyclic-dimer hydrolase from Arthrobacter sp. KI72.

Kengo Yasuhira1, Yuki Uedo, Naoki Shibata, Seiji Negoro, Masahiro Takeo, Yoshiki Higuchi.   

Abstract

6-Aminohexanoate-cyclic-dimer hydrolase (EI) from Arthrobacter sp. KI72 was expressed in Escherichia coli and purified by anion-exchange chromatography. EI was crystallized by the sitting-drop vapour-diffusion method with sodium citrate as precipitant in imidazole buffer pH 8.0. The crystal is hexagonal, with unit-cell parameters a = b = 130.75, c = 58.23 A. Diffraction data were collected from native and mercury(II) dichloride-derivative crystals to resolutions of 1.90 and 2.06 A, respectively.

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Year:  2006        PMID： 17142898      PMCID： PMC2225356          DOI： 10.1107/S1744309106045076

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  8 in total

Review 1.  Biodegradation of nylon oligomers.

Authors:  S Negoro
Journal:  Appl Microbiol Biotechnol       Date:  2000-10       Impact factor: 4.813

2.  6-Aminohexanoic acid cyclic dimer hydrolase. A new cyclic amide hydrolase produced by Achromobacter guttatus KI74.

Authors:  S Kinoshita; S Negoro; M Muramatsu; V S Bisaria; S Sawada; H Okada
Journal:  Eur J Biochem       Date:  1977-11-01

3.  Construction of a plasmid vector for the regulatable high level expression of eukaryotic genes in Escherichia coli: an application to overproduction of chicken lysozyme.

Authors:  T Miki; T Yasukochi; H Nagatani; M Furuno; T Orita; H Yamada; T Imoto; T Horiuchi
Journal:  Protein Eng       Date:  1987 Aug-Sep

4.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

5.  Nylon oligomer degradation gene, nylC, on plasmid pOAD2 from a Flavobacterium strain encodes endo-type 6-aminohexanoate oligomer hydrolase: purification and characterization of the nylC gene product.

Authors:  S Kakudo; S Negoro; I Urabe; H Okada
Journal:  Appl Environ Microbiol       Date:  1993-11       Impact factor: 4.792

6.  High homology between 6-aminohexanoate-cyclic-dimer hydrolases of Flavobacterium and Pseudomonas strains.

Authors:  K Tsuchiya; S Fukuyama; N Kanzaki; K Kanagawa; S Negoro; H Okada
Journal:  J Bacteriol       Date:  1989-06       Impact factor: 3.490

7.  Purification and characterization of 6-aminohexanoic-acid-oligomer hydrolase of Flavobacterium sp. Ki72.

Authors:  S Kinoshita; T Terada; T Taniguchi; Y Takene; S Masuda; N Matsunaga; H Okada
Journal:  Eur J Biochem       Date:  1981-06-01

8.  Plasmid-determined enzymatic degradation of nylon oligomers.

Authors:  S Negoro; T Taniguchi; M Kanaoka; H Kimura; H Okada
Journal:  J Bacteriol       Date:  1983-07       Impact factor: 3.490
  8 in total
  2 in total

1.  X-ray crystallographic analysis of the 6-aminohexanoate cyclic dimer hydrolase: catalytic mechanism and evolution of an enzyme responsible for nylon-6 byproduct degradation.

Authors:  Kengo Yasuhira; Naoki Shibata; Go Mongami; Yuki Uedo; Yu Atsumi; Yasuyuki Kawashima; Atsushi Hibino; Yusuke Tanaka; Young-Ho Lee; Dai-ichiro Kato; Masahiro Takeo; Yoshiki Higuchi; Seiji Negoro
Journal:  J Biol Chem       Date:  2009-11-03       Impact factor: 5.157

Review 2.  Plastics: Environmental and Biotechnological Perspectives on Microbial Degradation.

Authors:  Dominik Danso; Jennifer Chow; Wolfgang R Streit
Journal:  Appl Environ Microbiol       Date:  2019-09-17       Impact factor: 4.792
  2 in total

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