Literature DB >> 17088479

Overexpression and initial characterization of the chromosomal aminoglycoside 3'-O-phosphotransferase APH(3')-IIb from Pseudomonas aeruginosa.

Mariana Hainrichson1, Orit Yaniv, Marina Cherniavsky, Igor Nudelman, Dalia Shallom-Shezifi, Sima Yaron, Timor Baasov.   

Abstract

The chromosomal gene aph(3')-IIb, encoding an aminoglycoside 3'-phosphotransferase in Pseudomonas aeruginosa, was cloned and overexpressed in Escherichia coli. The APH(3')-IIb enzyme was purified as a monomer in a two-step procedure and was shown to phosphorylate its substrates at the C-3'-OH position, with kcat/Km values of 0.4x10(4) to 36x10(4) M-1 s-1.

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Year:  2006        PMID： 17088479      PMCID： PMC1797760          DOI： 10.1128/AAC.01034-06

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  11 in total

Review 1.  Phosphoryl transfer by aminoglycoside 3'-phosphotransferases and manifestation of antibiotic resistance.

Authors:  Choonkeun Kim; Shahriar Mobashery
Journal:  Bioorg Chem       Date:  2004-12-15       Impact factor: 5.275

2.  Branched aminoglycosides: biochemical studies and antibacterial activity of neomycin B derivatives.

Authors:  Mariana Hainrichson; Varvara Pokrovskaya; Dalia Shallom-Shezifi; Micha Fridman; Valery Belakhov; Dina Shachar; Sima Yaron; Timor Baasov
Journal:  Bioorg Med Chem       Date:  2005-10-15       Impact factor: 3.641

3.  Sequence and characterization of a novel chromosomal aminoglycoside phosphotransferase gene, aph (3')-IIb, in Pseudomonas aeruginosa.

Authors:  H Hächler; P Santanam; F H Kayser
Journal:  Antimicrob Agents Chemother       Date:  1996-05       Impact factor: 5.191

4.  Hydrolysis of ATP by aminoglycoside 3'-phosphotransferases: an unexpected cost to bacteria for harboring an antibiotic resistance enzyme.

Authors:  Choonkeun Kim; Joo Young Cha; Honggao Yan; Sergei B Vakulenko; Shahriar Mobashery
Journal:  J Biol Chem       Date:  2006-01-03       Impact factor: 5.157

5.  The enhancement of PCR amplification by low molecular-weight sulfones.

Authors:  R Chakrabarti; C E Schutt
Journal:  Gene       Date:  2001-08-22       Impact factor: 3.688

6.  Purification and characterization of aminoglycoside 3'-phosphotransferase type IIa and kinetic comparison with a new mutant enzyme.

Authors:  J J Siregar; S A Lerner; S Mobashery
Journal:  Antimicrob Agents Chemother       Date:  1994-04       Impact factor: 5.191

7.  Purification, characterization, and investigation of the mechanism of aminoglycoside 3'-phosphotransferase type Ia.

Authors:  J J Siregar; K Miroshnikov; S Mobashery
Journal:  Biochemistry       Date:  1995-10-03       Impact factor: 3.162

8.  Broad spectrum aminoglycoside phosphotransferase type III from Enterococcus: overexpression, purification, and substrate specificity.

Authors:  G A McKay; P R Thompson; G D Wright
Journal:  Biochemistry       Date:  1994-06-07       Impact factor: 3.162

Review 9.  Aminoglycoside phosphotransferases: proteins, structure, and mechanism.

Authors:  G D Wright; P R Thompson
Journal:  Front Biosci       Date:  1999-01-01

10.  aph(3')-IIb, a gene encoding an aminoglycoside-modifying enzyme, is under the positive control of surrogate regulator HpaA.

Authors:  Lin Zeng; Shouguang Jin
Journal:  Antimicrob Agents Chemother       Date:  2003-12       Impact factor: 5.191
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  3 in total

Review 1.  Pseudomonas aeruginosa: pathogenesis, virulence factors, antibiotic resistance, interaction with host, technology advances and emerging therapeutics.

Authors:  Shugang Qin; Wen Xiao; Chuanmin Zhou; Qinqin Pu; Xin Deng; Lefu Lan; Haihua Liang; Xiangrong Song; Min Wu
Journal:  Signal Transduct Target Ther       Date:  2022-06-25

2.  Novel thermostable antibiotic resistance enzymes from the Atlantis II Deep Red Sea brine pool.

Authors:  Ali H A Elbehery; David J Leak; Rania Siam
Journal:  Microb Biotechnol       Date:  2016-12-22       Impact factor: 5.813

3.  Antibiotic resistance determinants in a Pseudomonas putida strain isolated from a hospital.

Authors:  Lázaro Molina; Zulema Udaondo; Estrella Duque; Matilde Fernández; Carlos Molina-Santiago; Amalia Roca; Mario Porcel; Jesús de la Torre; Ana Segura; Patrick Plesiat; Katy Jeannot; Juan-Luis Ramos
Journal:  PLoS One       Date:  2014-01-17       Impact factor: 3.240
  3 in total

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