Yuanyuan Liu1, Chin-Ying Stephen Hsu. 1. Department of Preventive Dentistry, Faculty of Dentistry, National University of Singapore, 5 Lower Kent Ridge Road, Singapore 119074, Republic of Singapore.
Abstract
UNLABELLED: Preventive effects of lasers on enamel caries have been documented in the past few decades. However, its specific mechanism remains uncertain. OBJECTIVES: To evaluate the laser-induced change of enamel compositions, including carbonate and organic matters using Fourier-transform Raman spectroscopy (FT-Raman). METHODS: Twelve windows (1 mm x 1 mm), created on six defect-free primary incisors, were characterized by FT-Raman microscopy (1024 nm) before and after Er:YAG laser treatment (Fidelis) with 5.1 J/cm(2)-2 Hz-5s. To assess the statistical significance of laser effects, Raman peaks assigned to nu(1) phosphate, type-A/B carbonates, and organic matters were evaluated with the paired-samples t-test. RESULTS: The standardized intensity of type B carbonate decreased significantly from 0.117 to 0.106 (p=0.029), whereas the standardized intensity of carbonate A remained unchanged (p=0.467). Related to organic matters, the standardized intensity of peaks at 2940 cm(-1) and in the ranges of 1200-1600 cm(-1) decreased significantly, with p=0.005 and p<0.001, respectively. Revealing enamel crystallinity, the bandwidth of nu(1) phosphate on lased surfaces appeared to be unaltered after laser treatment (p=0.477). CONCLUSIONS: Laser treatment may provide caries-preventive effect on enamel through reduction of carbonate and modification of organic matters.
UNLABELLED: Preventive effects of lasers on enamel caries have been documented in the past few decades. However, its specific mechanism remains uncertain. OBJECTIVES: To evaluate the laser-induced change of enamel compositions, including carbonate and organic matters using Fourier-transform Raman spectroscopy (FT-Raman). METHODS: Twelve windows (1 mm x 1 mm), created on six defect-free primary incisors, were characterized by FT-Raman microscopy (1024 nm) before and after Er:YAG laser treatment (Fidelis) with 5.1 J/cm(2)-2 Hz-5s. To assess the statistical significance of laser effects, Raman peaks assigned to nu(1) phosphate, type-A/B carbonates, and organic matters were evaluated with the paired-samples t-test. RESULTS: The standardized intensity of type B carbonate decreased significantly from 0.117 to 0.106 (p=0.029), whereas the standardized intensity of carbonate A remained unchanged (p=0.467). Related to organic matters, the standardized intensity of peaks at 2940 cm(-1) and in the ranges of 1200-1600 cm(-1) decreased significantly, with p=0.005 and p<0.001, respectively. Revealing enamel crystallinity, the bandwidth of nu(1) phosphate on lased surfaces appeared to be unaltered after laser treatment (p=0.477). CONCLUSIONS: Laser treatment may provide caries-preventive effect on enamel through reduction of carbonate and modification of organic matters.
Authors: Mary A S de-Melo; Vanara F Passos; Jose J Alves; Eduardo B Barros; Sérgio L Santiago; Lidiany K A Rodrigues Journal: Lasers Med Sci Date: 2010-11-20 Impact factor: 3.161
Authors: Elza Maria de Sá Ferreira; Luis Eduardo Silva Soares; Héliton Spíndola Antunes; Sofia Takeda Uemura; Patrícia da Silva Barbosa; Hélio Augusto Salmon; Giselle Rodrigues de Sant'Anna Journal: Clin Oral Investig Date: 2015-12-22 Impact factor: 3.573