Elza Maria de Sá Ferreira1,2, Luis Eduardo Silva Soares3, Héliton Spíndola Antunes4,5,6, Sofia Takeda Uemura7, Patrícia da Silva Barbosa1, Hélio Augusto Salmon2, Giselle Rodrigues de Sant'Anna1. 1. School of Dentistry, Universidade Cruzeiro do Sul (Unicsul), R. Galvão Bueno, 868, Liberdade, São Paulo, SP, CEP- 01506-000, Brazil. 2. Clínicas Oncológicas Integradas (COI), Oral Medicine Service, Av. das Américas, 6205, loja E, Barra da Tijuca, Rio de Janeiro, RJ, CEP- 22793-080, Brazil. 3. Research and Development Institute, IP&D, Laboratory of Biomedical Vibracional Spectroscopy, LEVB, Universidade do Vale do Paraíba (UNIVAP), São José dos Campos, SP, Brazil. lesoares@univap.br. 4. Clínicas Oncológicas Integradas (COI), Radiotherapy, Av. das Américas, 6205, loja E, Barra da Tijuca, Rio de Janeiro, RJ, CEP- 22793-080, Brazil. 5. Instituto Nacional de Câncer (INCA), Cordination of Clinical Research, Rua André Cavalcante, 37, 2º andar, Centro, Rio de Janeiro, RJ, CEP- 20231-050, Brazil. 6. School of Dentistry, Universidade Federal Fluminense (UFF), R. Mário Santos Braga, 28, Campos do Valonguinho,Centro, Niterói, Rio de Janeiro, RJ, CEP- 24020-150, Brazil. 7. Faculdade de Odontologia Uniararas (FHO-Uniararas), Cordination of Dental School, Av. Dr. Maximiliano Baruto, 500, Araras, SP, CEP- 13607-339, Brazil.
Abstract
OBJECTIVES: This study evaluated the effects of radiotherapy on the composition of deciduous teeth enamel using micro-energy-dispersive X-ray fluorescence and Fourier transform Raman spectroscopy before and after a pH cycling process. MATERIALS AND METHODS: Ten deciduous molars were sectioned and divided into two groups (n = 10). The radiotherapy group (RT) was irradiated with 54 Gy at 2 Gy/day, 5 days per week for 5 weeks and 2 days, and the normal group (N) was not irradiated. The RT group was evaluated before radiotherapy (RTb), after radiotherapy (RTa), and after radiotherapy and pH cycling (RTc). The normal group was evaluated before (N) and after pH cycling (Nc). The weight percentage (wt%) of calcium (Ca), phosphorus (P), and organic content; the Ca/P ratio; and the integrated area of the Raman bands relative to the organic, carbonate, and phosphate contents were also evaluated. RESULTS: The exclusive use of RT reduced the organic content of the enamel (p = 0.000). The RTc group exhibited a decrease in P wt% (p = 0.016), an increase in the Ca/P ratio (p = 0.000), and a reduction in the integrated area of the phosphate band (p = 0.046). An increase in the Ca/P ratio (p = 0.000) and a reduction in the areas of the carbonate and phosphate bands were found in the RTb/RTc treatments. CONCLUSIONS: RT application at a therapeutic dose reduced the organic content of the deciduous enamel. CLINICAL RELEVANCE: Preventive measures should be included in the patient treatment protocol because of RT-induced chemical changes to the deciduous enamel.
OBJECTIVES: This study evaluated the effects of radiotherapy on the composition of deciduous teeth enamel using micro-energy-dispersive X-ray fluorescence and Fourier transform Raman spectroscopy before and after a pH cycling process. MATERIALS AND METHODS: Ten deciduous molars were sectioned and divided into two groups (n = 10). The radiotherapy group (RT) was irradiated with 54 Gy at 2 Gy/day, 5 days per week for 5 weeks and 2 days, and the normal group (N) was not irradiated. The RT group was evaluated before radiotherapy (RTb), after radiotherapy (RTa), and after radiotherapy and pH cycling (RTc). The normal group was evaluated before (N) and after pH cycling (Nc). The weight percentage (wt%) of calcium (Ca), phosphorus (P), and organic content; the Ca/P ratio; and the integrated area of the Raman bands relative to the organic, carbonate, and phosphate contents were also evaluated. RESULTS: The exclusive use of RT reduced the organic content of the enamel (p = 0.000). The RTc group exhibited a decrease in P wt% (p = 0.016), an increase in the Ca/P ratio (p = 0.000), and a reduction in the integrated area of the phosphate band (p = 0.046). An increase in the Ca/P ratio (p = 0.000) and a reduction in the areas of the carbonate and phosphate bands were found in the RTb/RTc treatments. CONCLUSIONS: RT application at a therapeutic dose reduced the organic content of the deciduous enamel. CLINICAL RELEVANCE: Preventive measures should be included in the patient treatment protocol because of RT-induced chemical changes to the deciduous enamel.
Entities:
Keywords:
Deciduous enamel; Energy-dispersive X-ray spectroscopy; Fourier transform Raman spectroscopy; Head and neck cancer; Radiotherapy
Authors: Carolina Steiner-Oliveira; Lidiany K A Rodrigues; Luís E S Soares; Airton A Martin; Denise M Zezell; Marinês Nobre-dos-Santos Journal: Dent Mater J Date: 2006-09 Impact factor: 2.102
Authors: Aline Lima da Silva Deboni; Adelmo José Giordani; Nilza Nelly Fontana Lopes; Rodrigo Souza Dias; Roberto Araujo Segreto; Siri Beier Jensen; Helena Regina Comodo Segreto Journal: Support Care Cancer Date: 2012-03-13 Impact factor: 3.603