AIM: To investigate chromosome 8 numerical aberrations, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histopathological characteristics of gastric tumors. METHODS: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immunostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed. RESULTS: All the cases showed chromosome 8 aneuploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level of chromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal-type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm. CONCLUSION: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic pathways.
AIM: To investigate chromosome 8 numerical aberrations, C-MYC oncogene alterations and its expression in gastric cancer and to correlate these findings with histopathological characteristics of gastric tumors. METHODS: Specimens were collected surgically from seven patients with gastric adenocarcinomas. Immunostaining for C-MYC and dual-color fluorescence in situ hybridization (FISH) for C-MYC gene and chromosome 8 centromere were performed. RESULTS: All the cases showed chromosome 8 aneuploidy and C-MYC amplification, in both the diffuse and intestinal histopathological types of Lauren. No significant difference (P < 0.05) was observed between the level of chromosome 8 ploidy and the site, stage or histological type of the adenocarcinomas. C-MYC high amplification, like homogeneously stained regions (HSRs) and double minutes (DMs), was observed only in the intestinal-type. Structural rearrangement of C-MYC, like translocation, was observed only in the diffuse type. Regarding C-MYC gene, a significant difference (P < 0.05) was observed between the two histological types. The C-MYC protein was expressed in all the studied cases. In the intestinal-type the C-MYC immunoreactivity was localized only in the nucleus and in the diffuse type in the nucleus and cytoplasm. CONCLUSION: Distinct patterns of alterations between intestinal and diffuse types of gastric tumors support the hypothesis that these types follow different genetic pathways.
Authors: E M Lima; J D Rissino; M L Harada; P P Assumpção; S Demachki; A C Guimarães; C Casartelli; M A C Smith; R R Burbano Journal: Braz J Med Biol Res Date: 2004-11-17 Impact factor: 2.590
Authors: Carlo Valentino; Samantha Kendrick; Nathalie Johnson; Randy Gascoyne; Wing C Chan; Dennis Weisenburger; Rita Braziel; James R Cook; Raymond Tubbs; Elias Campo; Andreas Rosenwald; German Ott; Jan Delabie; Elaine Jaffe; Wenjun Zhang; Patrick Brunhoeber; Hiro Nitta; Tom Grogan; Lisa Rimsza Journal: Am J Clin Pathol Date: 2013-02 Impact factor: 2.493
Authors: André S Khayat; Adriana C Guimarães; Danielle Q Calcagno; Aline D Seabra; Eleonidas M Lima; Mariana F Leal; Mário H G Faria; Silvia H B Rabenhorst; Paulo P Assumpção; Samia Demachki; Marília A C Smith; Rommel R Burbano Journal: BMC Gastroenterol Date: 2009-07-20 Impact factor: 3.067