Literature DB >> 1703066

A one-step efficient and specific non-radioactive non-fluorescent method for in situ hybridization of banded chromosomes.

F R Zhang1, R Heilig, G Thomas, A Aurias.   

Abstract

A non-radioactive method for in situ hybridization of cosmid probes to metaphase chromosomes is described. Two procedures are involved: (i) hybridization with a cosmid probe labelled by nick translation in the presence of digoxigenin dUTP. The signal is visualized by an alkaline phosphatase conjugated antibody. (ii) FPG banding of the chromosomes. The steps involved in these two procedures are combined in an order which allows simultaneous observation of the banding pattern and the hybridization signal. The metaphases can thus be analysed after a single photographic step. This technique is considerably simpler than the method used previously.

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Year:  1990        PMID： 1703066     DOI： 10.1007/bf01726696

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  8 in total

1.  Mapping of single-copy DNA sequences on human chromosomes by in situ hybridization with biotinylated probes: enhancement of detection sensitivity by intensified-fluorescence digital-imaging microscopy.

Authors:  E Viegas-Pequignot; B Dutrillaux; H Magdelenat; M Coppey-Moisan
Journal:  Proc Natl Acad Sci U S A       Date:  1989-01       Impact factor: 11.205

2.  New Giemsa method for the differential staining of sister chromatids.

Authors:  P Perry; S Wolff
Journal:  Nature       Date:  1974-09-13       Impact factor: 49.962

3.  [A new technic of analysis of the human karyotype].

Authors:  B Dutrillaux; J Lejeune
Journal:  C R Acad Hebd Seances Acad Sci D       Date:  1971-05-17

4.  DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome.

Authors:  M G Mattei; N Philip; E Passage; J P Moisan; J L Mandel; J F Mattei
Journal:  Hum Genet       Date:  1985       Impact factor: 4.132

5.  Immunological method for mapping genes on Drosophila polytene chromosomes.

Authors:  P R Langer-Safer; M Levine; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

6.  Localization of the human insulin gene to the distal end of the short arm of chromosome 11.

Authors:  M E Harper; A Ullrich; G F Saunders
Journal:  Proc Natl Acad Sci U S A       Date:  1981-07       Impact factor: 11.205

7.  Novel non-isotopic in situ hybridization technique detects small (1 Kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes.

Authors:  J A Garson; J A van den Berghe; J T Kemshead
Journal:  Nucleic Acids Res       Date:  1987-06-25       Impact factor: 16.971

8.  Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.

Authors:  D Pinkel; T Straume; J W Gray
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205
  8 in total
  7 in total

1.  A case of apparent trisomy 21 without the Down's syndrome phenotype.

Authors:  D Avramopoulos; I Kennerknecht; G Barbi; D Eckert; J M Delabar; C Maunoury; A Hallberg; M B Petersen
Journal:  J Med Genet       Date:  1997-07       Impact factor: 6.318

2.  Screening of microdeletions of chromosome 20 in patients with Alagille syndrome.

Authors:  C Desmaze; J F Deleuze; A M Dutrillaux; G Thomas; M Hadchouel; A Aurias
Journal:  J Med Genet       Date:  1992-04       Impact factor: 6.318

3.  Simultaneous visualization of chromosome bands and hybridization signal using colloidal-gold labeling in electron microscopy.

Authors:  R Fetni; R Drouin; N Lemieux; P E Messier; C L Richer
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-01       Impact factor: 11.205

4.  Deleted chromosome 20 from a patient with Alagille syndrome isolated in a cell hybrid through leucine transport selection: study of three candidate genes.

Authors:  J F Deleuze; S Dhorne; J Hazan; E Borghi; N Raynaud; N Pollet; M Meunier-Rotival; J Deschatrette; D Alagille; M Hadchouel
Journal:  Mamm Genome       Date:  1994-11       Impact factor: 2.957

5.  The mapping of transgenes by fluorescence in situ hybridization on G-banded mouse chromosomes.

Authors:  Y P Shi; T T Huang; E J Carlson; C J Epstein
Journal:  Mamm Genome       Date:  1994-06       Impact factor: 2.957

6.  Detection of deletions and cryptic translocations in Miller-Dieker syndrome by in situ hybridization.

Authors:  A Kuwano; S A Ledbetter; W B Dobyns; B S Emanuel; D H Ledbetter
Journal:  Am J Hum Genet       Date:  1991-10       Impact factor: 11.025

7.  Sex chromosome polymorphism and heterogametic males revealed by two cloned DNA probes in the ZW/ZZ fish Leporinus elongatus.

Authors:  I Nakayama; F Foresti; R Tewari; M Schartl; D Chourrout
Journal:  Chromosoma       Date:  1994-03       Impact factor: 4.316
  7 in total

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