Literature DB >> 8043947

The mapping of transgenes by fluorescence in situ hybridization on G-banded mouse chromosomes.

Y P Shi1, T T Huang, E J Carlson, C J Epstein.   

Abstract

A highly sensitive method for the mapping of transgenes and other genes in the mouse genome is described. This technique combines high-resolution G-banding and fluorescence in situ hybridization (FISH) with either biotin/avidin-FITC or digoxigenin-anti-digoxigenin-FITC, the latter being the more sensitive. Banding patterns are obtained with trypsin/Giemsa-treated slides, and sensitivity is greatly increased by the use of mouse Cot-1 DNA. With this protocol, four different 14.5-kb human Cu/Zn-superoxide dismutase transgene insertions ranging in copy number from 2 to 8 have been localized to four different mouse chromosomes. The utility and sensitivity of this procedure were verified with a Chromosome (Chr) 16-specific cosmid probe, H22, as well as with the mapping of a high-copy-number human beta-amyloid/A4 transgene.

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Year:  1994        PMID： 8043947     DOI： 10.1007/bf00356551

Source DB:  PubMed          Journal:  Mamm Genome        ISSN： 0938-8990            Impact factor:   2.957


  22 in total

1.  A one-step efficient and specific non-radioactive non-fluorescent method for in situ hybridization of banded chromosomes.

Authors:  F R Zhang; R Heilig; G Thomas; A Aurias
Journal:  Chromosoma       Date:  1990-10       Impact factor: 4.316

2.  High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones.

Authors:  P Lichter; C J Tang; K Call; G Hermanson; G A Evans; D Housman; D C Ward
Journal:  Science       Date:  1990-01-05       Impact factor: 47.728

3.  Rapid detection of human chromosome 21 aberrations by in situ hybridization.

Authors:  P Lichter; T Cremer; C J Tang; P C Watkins; L Manuelidis; D C Ward
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

4.  Karyotyping.

Authors:  R G Worton; C Duff
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

5.  Measurement by quantitative PCR of changes in HPRT, PGK-1, PGK-2, APRT, MTase, and Zfy gene transcripts during mouse spermatogenesis.

Authors:  J Singer-Sam; M O Robinson; A R Bellvé; M I Simon; A D Riggs
Journal:  Nucleic Acids Res       Date:  1990-03-11       Impact factor: 16.971

6.  An improved method for preparing G-banded chromosomes from mouse peripheral blood.

Authors:  M T Davisson; E C Akeson
Journal:  Cytogenet Cell Genet       Date:  1987

7.  Location of the mouse complement factor H gene (cfh) by FISH analysis and replication R-banding.

Authors:  Y Matsuda; Y N Harada; S Natsuume-Sakai; K Lee; T Shiomi; V M Chapman
Journal:  Cytogenet Cell Genet       Date:  1992

8.  Chromosomal localization of the ovine beta-casein gene by non-isotopic in situ hybridization and R-banding.

Authors:  H Hayes; E Petit; N Lemieux; B Dutrillaux
Journal:  Cytogenet Cell Genet       Date:  1992

9.  Fine mapping of the Huntington disease linked D4S10 locus by non-radioactive in situ hybridization.

Authors:  J E Landegent; N Jansen in de Wal; Y M Fisser-Groen; E Bakker; M van der Ploeg; P L Pearson
Journal:  Hum Genet       Date:  1986-08       Impact factor: 4.132

10.  Overproduction of human Cu/Zn-superoxide dismutase in transfected cells: extenuation of paraquat-mediated cytotoxicity and enhancement of lipid peroxidation.

Authors:  O Elroy-Stein; Y Bernstein; Y Groner
Journal:  EMBO J       Date:  1986-03       Impact factor: 11.598
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  8 in total

1.  Ts65Dn mouse, a Down syndrome model, exhibits elevated myo-inositol in selected brain regions and peripheral tissues.

Authors:  H U Shetty; R J Siarey; Z Galdzicki; J Stoll; S I Rapoport
Journal:  Neurochem Res       Date:  2000-04       Impact factor: 3.996

2.  Fine mapping of the alpha-NAC gene (Naca) to the distal end (D2-D3) of mouse chromosome 10.

Authors:  W V Yotov; R St-Arnaud
Journal:  Mamm Genome       Date:  1996-03       Impact factor: 2.957

3.  Ts1Cje, a partial trisomy 16 mouse model for Down syndrome, exhibits learning and behavioral abnormalities.

Authors:  H Sago; E J Carlson; D J Smith; J Kilbridge; E M Rubin; W C Mobley; C J Epstein; T T Huang
Journal:  Proc Natl Acad Sci U S A       Date:  1998-05-26       Impact factor: 11.205

4.  Analysis of transgene integration sites in transgenic pigs by fluorescence in situ hybridization.

Authors:  H W Kuipers; G A Langford; D J White
Journal:  Transgenic Res       Date:  1997-07       Impact factor: 2.788

5.  Mouse molecular cytogenetic resource: 157 BACs link the chromosomal and genetic maps.

Authors:  J R Korenberg; X N Chen; K L Devon; D Noya; M L Oster-Granite; B W Birren
Journal:  Genome Res       Date:  1999-05       Impact factor: 9.043

6.  Rapid fluorescence in situ hybridization on interphasic nuclei to discriminate between homozygous and heterozygous transgenic mice.

Authors:  D Paris; K Toyama; A Mégarbané; P M Casanova; P M Sinet; J London
Journal:  Transgenic Res       Date:  1996-11       Impact factor: 2.788

7.  Overexpression of SOD1 in transgenic rats protects vulnerable neurons against ischemic damage after global cerebral ischemia and reperfusion.

Authors:  P H Chan; M Kawase; K Murakami; S F Chen; Y Li; B Calagui; L Reola; E Carlson; C J Epstein
Journal:  J Neurosci       Date:  1998-10-15       Impact factor: 6.167

8.  Down's syndrome suppression of tumour growth and the role of the calcineurin inhibitor DSCR1.

Authors:  Kwan-Hyuck Baek; Alexander Zaslavsky; Ryan C Lynch; Carmella Britt; Yoshiaki Okada; Richard J Siarey; M William Lensch; In-Hyun Park; Sam S Yoon; Takashi Minami; Julie R Korenberg; Judah Folkman; George Q Daley; William C Aird; Zygmunt Galdzicki; Sandra Ryeom
Journal:  Nature       Date:  2009-05-20       Impact factor: 49.962
  8 in total

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