Literature DB >> 17012807

Crystallization and preliminary X-ray crystallographic studies of pig heart carbonyl reductase.

Ken-ichi Aoki1, Nobutada Tanaka, Shuhei Ishikura, Naoko Araki, Yorishige Imamura, Akira Hara, Kazuo T Nakamura.   

Abstract

Pig heart carbonyl reductase (PHCR), which belongs to the short-chain dehydrogenase/reductase (SDR) family, has been crystallized by the hanging-drop vapour-diffusion method. Two crystal forms (I and II) have been obtained in the presence of NADPH. Form I crystals belong to the tetragonal space group P4(2), with unit-cell parameters a = b = 109.61, c = 94.31 A, and diffract to 1.5 A resolution. Form II crystals belong to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 120.10, c = 147.00 A, and diffract to 2.2 A resolution. Both crystal forms are suitable for X-ray structure analysis at high resolution.

Entities:  

Mesh:

Substances:

Year:  2006        PMID: 17012807      PMCID: PMC2225176          DOI: 10.1107/S1744309106037535

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN: 1744-3091


  20 in total

1.  Porcine carbonyl reductase. structural basis for a functional monomer in short chain dehydrogenases/reductases.

Authors:  D Ghosh; M Sawicki; V Pletnev; M Erman; S Ohno; S Nakajin; W L Duax
Journal:  J Biol Chem       Date:  2001-03-08       Impact factor: 5.157

2.  Data processing.

Authors:  M G Rossmann; C G van Beek
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1999-10

Review 3.  Carbonyl reductase.

Authors:  G L Forrest; B Gonzalez
Journal:  Chem Biol Interact       Date:  2000-12-01       Impact factor: 5.192

4.  Characterization of pulmonary carbonyl reductase of mouse and guinea pig.

Authors:  T Nakayama; K Yashiro; Y Inoue; K Matsuura; H Ichikawa; A Hara; H Sawada
Journal:  Biochim Biophys Acta       Date:  1986-06-19

5.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

Review 6.  Steroid dehydrogenase structures, mechanism of action, and disease.

Authors:  W L Duax; D Ghosh; V Pletnev
Journal:  Vitam Horm       Date:  2000       Impact factor: 3.421

7.  Crystal structures of the binary and ternary complexes of 7 alpha-hydroxysteroid dehydrogenase from Escherichia coli.

Authors:  N Tanaka; T Nonaka; T Tanabe; T Yoshimoto; D Tsuru; Y Mitsui
Journal:  Biochemistry       Date:  1996-06-18       Impact factor: 3.162

8.  Human carbonyl reductase. Nucleotide sequence analysis of a cDNA and amino acid sequence of the encoded protein.

Authors:  B Wermuth; K M Bohren; G Heinemann; J P von Wartburg; K H Gabbay
Journal:  J Biol Chem       Date:  1988-11-05       Impact factor: 5.157

Review 9.  Short-chain dehydrogenases/reductases (SDR).

Authors:  H Jörnvall; B Persson; M Krook; S Atrian; R Gonzàlez-Duarte; J Jeffery; D Ghosh
Journal:  Biochemistry       Date:  1995-05-09       Impact factor: 3.162

10.  An unbiased cell morphology-based screen for new, biologically active small molecules.

Authors:  Masahiro Tanaka; Raynard Bateman; Daniel Rauh; Eugeni Vaisberg; Shyam Ramachandani; Chao Zhang; Kirk C Hansen; Alma L Burlingame; Jay K Trautman; Kevan M Shokat; Cynthia L Adams
Journal:  PLoS Biol       Date:  2005-04-05       Impact factor: 8.029

View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.