| Literature DB >> 16996056 |
Zhen F Fang1, Hui Gai, You Z Huang, Shan G Li, Xue J Chen, Jian J Shi, Li Wu, Ailian Liu, Ping Xu, Hui Z Sheng.
Abstract
Embryonic stem cells were isolated from rabbit blastocysts derived from fertilization (conventional rbES cells), parthenogenesis (pES cells) and nuclear transfer (ntES cells), and propagated in a serum-free culture system. Rabbit ES (rbES) cells proliferated for a prolonged time in an undifferentiated state and maintained a normal karyotype. These cells grew in a monolayer with a high nuclear/cytoplasm ratio and contained a high level of alkaline phosphate activity. In addition, rbES cells expressed the pluripotent marker Oct-4, as well as EBAF2, FGF4, TDGF1, but not antigens recognized by antibodies against SSEA-1, SSEA-3, SSEA-4, TRA-1-10 and TRA-1-81. All 3 types of ES cells formed embryoid bodies and generated teratoma that contained tissue types of all three germ layers. rbES cells exhibited a high cloning efficiency, were genetically modified readily and were used as nuclear donors to generate a viable rabbit through somatic cell nuclear transfer. In combination with genetic engineering, the ES cell technology should facilitate the creation of new rabbit lines.Entities:
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Year: 2006 PMID： 16996056 DOI： 10.1016/j.yexcr.2006.08.013
Source DB: PubMed Journal: Exp Cell Res ISSN： 0014-4827 Impact factor: 3.905