| Literature DB >> 26579970 |
Fuliang Du1,2,3, Chien-Hong Chen2,3, Yi Li1, Yeshu Hu1, Li-You An1, Lan Yang4, Jifeng Zhang5, Y Eugene Chen5, Jie Xu2,5.
Abstract
The rabbit is a useful animal model for regenerative medicine. We previously developed pluripotent rabbit embryonic stem cell (rbESC) lines using fresh embryos. We also successfully cryopreserved rabbit embryos by vitrification. In the present work, we combined these two technologies to derive rbESCs using vitrified-thawed (V/T) embryos. We demonstrate that V/T blastocysts (BLs) can be used to derive pluripotent rbESCs with efficiencies comparable to those using fresh BLs. These ESCs are undistinguishable from the ones derived from fresh embryos. We tested the developmental capacity of rbESCs derived from V/T embryos by BL injection experiments and produced chimeric kits. Our work adds cryopreservation to the toolbox of rabbit stem cell research and applications and will greatly expand the available research materials for regenerative medicine in a clinically relevant animal model.Entities:
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Year: 2015 PMID： 26579970 PMCID： PMC4677569 DOI： 10.1089/cell.2015.0044
Source DB: PubMed Journal: Cell Reprogram ISSN： 2152-4971 Impact factor: 1.987