Literature DB >> 1699112

An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels.

A M Gonçalves1, N S Nehme, C M Morel.   

Abstract

A simple protocol is described for the silver staining of polyacrylamide gradient gels used for the separation of restriction fragments of kinetoplast DNA [schizodeme analysis of trypanosomatids (Morel et al., 1980)]. The method overcomes the problems of non-uniform staining and strong background color which are frequently encountered when conventional protocols for silver staining of linear gels are applied to gradient gels. The method described has proven to be of general applicability for DNA, RNA and protein separations in gradient gels.

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Year:  1990        PMID： 1699112     DOI： 10.1590/s0074-02761990000100017

Source DB:  PubMed          Journal:  Mem Inst Oswaldo Cruz        ISSN： 0074-0276            Impact factor:   2.743


  4 in total

1.  Schizodeme and zymodeme analysis of trypanosomes of the subgenus Schizotrypanum from the bat.

Authors:  L F Teixeira; A M Gonçalves; A J Romanha; M Steindel; A S Pinto
Journal:  Parasitol Res       Date:  1993       Impact factor: 2.289

2.  Cysteine proteinases from promastigotes of Leishmania (Viannia) braziliensis.

Authors:  Karina M Rebello; Luzia M C Côrtes; Bernardo A S Pereira; Bernardo M O Pascarelli; Suzana Côrte-Real; Léa C Finkelstein; Rosa T Pinho; Claudia M d'Avila-Levy; Carlos R Alves
Journal:  Parasitol Res       Date:  2009-09-24       Impact factor: 2.289

3.  Participation of heparin binding proteins from the surface of Leishmania (Viannia) braziliensis promastigotes in the adhesion of parasites to Lutzomyia longipalpis cells (Lulo) in vitro.

Authors:  Luzia Monteiro de Castro Côrtes; Mirian Claudia de Souza Pereira; Franklin Souza da Silva; Bernardo Acácio Santini Pereira; Francisco Odêncio de Oliveira Junior; Renata Oliveira de Araújo Soares; Reginaldo Peçanha Brazil; Leny Toma; Carolina Meloni Vicente; Helena Bonciani Nader; Maria de Fátima Madeira; Felio J Bello; Carlos Roberto Alves
Journal:  Parasit Vectors       Date:  2012-07-17       Impact factor: 3.876

4.  Evaluation of the genetic polymorphism of Plasmodium falciparum P126 protein (SERA or SERP) and its influence on naturally acquired specific antibody responses in malaria-infected individuals living in the Brazilian Amazon.

Authors:  Lilian Rose Pratt-Riccio; Selma Sallenave-Sales; Joseli de Oliveira-Ferreira; Bruno T da Silva; Monick Lindenmeyer Guimarães; Fátima Santos; Thatiane S de Simone; Mariza G Morgado; Salvatore G de Simone; Maria de Fátima Ferreira-Da-Cruz; Cláudio T Daniel-Ribeiro; Mariano G Zalis; Daniel Camus; Dalma M Banic
Journal:  Malar J       Date:  2008-07-30       Impact factor: 2.979
  4 in total

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