Literature DB >> 8415566

Schizodeme and zymodeme analysis of trypanosomes of the subgenus Schizotrypanum from the bat.

L F Teixeira1, A M Gonçalves, A J Romanha, M Steindel, A S Pinto.   

Abstract

Two stocks and nine clones of trypanosomes of the subgenus Schizotrypanum were analyzed based on their electrophoretic pattern of EcoR1 digestion products of kinetoplast DNA (k-DNA) minicircles (schizodeme) and isoenzymes (zymodeme). The trypanosome stocks were isolated from Phyllostomus hastatus bats collected in different locations in Minas Gerais State, Brazil. The k-DNA pattern showed major variations between stocks and microvariations among the clones. Furthermore, in one of several cloning attempts, two different populations could be isolated. Among the six enzymes studied, glucose phosphate isomerase presented different patterns for stocks and clones. No similarity was found among bat trypanosomes and Trypanosoma (Schizotrypanum) cruzi standard zymodemes. This shows that bat trypanosomes are distinct from T. (S.) cruzi.

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Year:  1993        PMID： 8415566     DOI： 10.1007/bf00931591

Source DB:  PubMed          Journal:  Parasitol Res        ISSN： 0932-0113            Impact factor:   2.289


  12 in total

1.  An improved silver staining procedure for schizodeme analysis in polyacrylamide gradient gels.

Authors:  A M Gonçalves; N S Nehme; C M Morel
Journal:  Mem Inst Oswaldo Cruz       Date:  1990 Jan-Mar       Impact factor: 2.743

2.  Isoenzyme patterns of cultured Trypanosoma cruzi: changes after prolonged subculture.

Authors:  A J Romanha; A A da Silva Pereira; E Chiari; V Kilgour
Journal:  Comp Biochem Physiol B       Date:  1979

3.  Stability of isoenzyme and kinetoplast DNA (k-DNA) patterns in successively cloned Trypanosoma cruzi populations.

Authors:  M L Gomes; A J Romanha; A M Gonçalves; E Chiari
Journal:  Mem Inst Oswaldo Cruz       Date:  1991 Oct-Dec       Impact factor: 2.743

4.  Biochemical characterization of some species of Trypansoma (Schizotrypanum) from bats (Microchiroptera).

Authors:  J R Baker; M A Miles; D G Godfrey; T V Barrett
Journal:  Am J Trop Med Hyg       Date:  1978-05       Impact factor: 2.345

5.  Characterization of pathogenic trypanosomatidae by restriction endonuclease fingerprinting of kinetoplast DNA minicircles.

Authors:  C Morel; L Simpson
Journal:  Am J Trop Med Hyg       Date:  1980-09       Impact factor: 2.345

6.  Further enzymic characters of Trypanosoma cruzi and their evaluation for strain identification.

Authors:  M A Miles; S M Lanham; A A de Souza; M Póvoa
Journal:  Trans R Soc Trop Med Hyg       Date:  1980       Impact factor: 2.184

7.  Trypanosoma cruzi: analysis of the population dynamics of heterogeneous mixtures.

Authors:  R W Finley; J A Dvorak
Journal:  J Protozool       Date:  1987-11

8.  Trypanosoma cruzi: inoculation schedules and re-isolation methods select individual strains from doubly infected mice, as demonstrated by schizodeme and zymodeme analyses.

Authors:  M P Deane; M A Sousa; N M Pereira; A M Gonçalves; H Momen; C M Morel
Journal:  J Protozool       Date:  1984-05

9.  Strains and clones of Trypanosoma cruzi can be characterized by pattern of restriction endonuclease products of kinetoplast DNA minicircles.

Authors:  C Morel; E Chiari; E P Camargo; D M Mattei; A J Romanha; L Simpson
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

10.  The identification by isoenzyme patterns of two distinct strain-groups of Trypanosoma cruzi, circulating independently in a rural area of Brazil.

Authors:  M A Miles; P J Toye; S C Oswald; D G Godfrey
Journal:  Trans R Soc Trop Med Hyg       Date:  1977       Impact factor: 2.184
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  1 in total

1.  Identification of bat trypanosomes from Minas Gerais state, Brazil, based on 18S rDNA and Cathepsin-L-like targets.

Authors:  Elaine Cristina Bento; César Gómez-Hernández; Lara Rocha Batista; Laís Anversa; André Luiz Pedrosa; Eliane Lages-Silva; Juan David Ramírez; Luis Eduardo Ramirez
Journal:  Parasitol Res       Date:  2018-01-16       Impact factor: 2.289
  1 in total

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