Literature DB >> 1696808

Serologically defined linear epitopes in the E2 envelope glycoprotein of Semliki Forest virus.

N Ariel1, S Lehrer, E Elhanaty, T Sabo, P Brodt, B Lachmi, D Katz, R Levin, H Grosfeld, B Velan.   

Abstract

A set of 41 overlapping peptides, representing the complete sequence of SFV-E2 protein were synthesized and analyzed in the ELISA test against murine anti-SFV sera. No single peptide was recognized by all antisera. Eight peptides were found to be highly reactive with hyperimmune anti-SFV sera. Six out of the eight peptide sequences coincide with the most hydrophilic regions of SFV-E2. Out of these, four peptides (amino acid positions 16-35, 61-80, 166-185, 286-305) that contain the least number of alphavirus conserved residues were selected. This panel constitutes the minimal number of peptides necessary and sufficient for specific recognition of hyperimmune mouse anti-SFV sera.

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Year:  1990        PMID： 1696808     DOI： 10.1007/bf01318358

Source DB:  PubMed          Journal:  Arch Virol        ISSN： 0304-8608            Impact factor:   2.574


  19 in total

1.  General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids.

Authors:  R A Houghten
Journal:  Proc Natl Acad Sci U S A       Date:  1985-08       Impact factor: 11.205

2.  A synthetic peptide for detecting antibodies to Epstein-Barr virus nuclear antigen in sera from patients with infectious mononucleosis.

Authors:  R S Smith; G Rhodes; J H Vaughan; C A Horwitz; J E Geltosky; A S Whalley
Journal:  J Infect Dis       Date:  1986-11       Impact factor: 5.226

3.  Simplified procedure for carrying out simultaneous multiple hydrogen fluoride cleavages of protected peptide resins.

Authors:  R A Houghten; M K Bray; S T Degraw; C J Kirby
Journal:  Int J Pept Protein Res       Date:  1986-06

4.  An evolutionary tree relating eight alphaviruses, based on amino-terminal sequences of their glycoproteins.

Authors:  J R Bell; R M Kinney; D W Trent; E G Strauss; J H Strauss
Journal:  Proc Natl Acad Sci U S A       Date:  1984-08       Impact factor: 11.205

5.  A simple method for displaying the hydropathic character of a protein.

Authors:  J Kyte; R F Doolittle
Journal:  J Mol Biol       Date:  1982-05-05       Impact factor: 5.469

6.  Nucleotide sequence of cdna coding for Semliki Forest virus membrane glycoproteins.

Authors:  H Garoff; A M Frischauf; K Simons; H Lehrach; H Delius
Journal:  Nature       Date:  1980-11-20       Impact factor: 49.962

7.  Antibodies to peptides detect new hepatitis B antigen: serological correlation with hepatocellular carcinoma.

Authors:  A M Moriarty; H Alexander; R A Lerner; G B Thornton
Journal:  Science       Date:  1985-01-25       Impact factor: 47.728

8.  Patterns of antibody recognition of selected conserved amino acid sequences from the HIV envelope in sera from different stages of HIV infection.

Authors:  A Shafferman; J Lennox; H Grosfeld; J Sadoff; R R Redfield; D S Burke
Journal:  AIDS Res Hum Retroviruses       Date:  1989-02       Impact factor: 2.205

9.  Diagnosis of AIDS by using a 12-amino acid peptide representing an immunodominant epitope of the human immunodeficiency virus.

Authors:  J W Gnann; P L Schwimmbeck; J A Nelson; A B Truax; M B Oldstone
Journal:  J Infect Dis       Date:  1987-08       Impact factor: 5.226

10.  Identification of distinct antigenic determinants on Semliki Forest virus by using monoclonal antibodies with different antiviral activities.

Authors:  W A Boere; T Harmsen; J Vinjé; B J Benaissa-Trouw; C A Kraaijeveld; H Snippe
Journal:  J Virol       Date:  1984-11       Impact factor: 5.103
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