| Literature DB >> 16962823 |
Yoshihiro Kita1, Takayo Ohto, Naonori Uozumi, Takao Shimizu.
Abstract
Phospholipase A(2) (PLA(2)) (EC 3.1.1.4) catalyzes hydrolysis of the sn-2 ester bond of glycerophospholipids. The enzyme is essential for the production of two classes of lipid mediators, fatty acid metabolites and lysophospholipid-related lipids, as well as being involved in the remodeling of membrane phospholipids. Among many mammalian PLA(2)s, cytosolic PLA(2)alpha (cPLA(2)alpha) plays a critical role in various physiological and pathophysiological conditions through generating lipid mediators. Here, we summarize the in vivo significance of cPLA(2)alpha, revealed from the phenotypes of cPLA(2)alpha-null mice, and properties of newly discovered cPLA(2) family enzymes. We also briefly introduce a quantitative lipidomics strategy using liquid chromatography-mass spectrometry, a powerful tool for the comprehensive analysis of lipid mediators.Entities:
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Year: 2006 PMID： 16962823 DOI： 10.1016/j.bbalip.2006.08.001
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002