Literature DB >> 1693630

Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae.

R Rossau1, M Duhamel, E Van Dyck, P Piot, H Van Heuverswyn.   

Abstract

The reliability of an rRNA-derived oligonucleotide probe for Neisseria gonorrhoeae was tested with 187 N. gonorrhoeae isolates, 81 Neisseria meningitidis isolates, and several strains of other bacterial species. The probe proved to be 100% specific and 100% sensitive. N. gonorrhoeae cells could also be reliably identified in contaminated cultures with the oligonucleotide probe. The 2.6-megadalton cryptic plasmid used as a probe for N. gonorrhoeae was shown to be less sensitive, detecting 179 of 181 N. gonorrhoeae isolates.

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Year:  1990        PMID： 1693630      PMCID： PMC267843          DOI： 10.1128/jcm.28.5.944-948.1990

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Homology of cryptic plasmid of Neisseria gonorrhoeae with plasmids from Neisseria meningitidis and Neisseria lactamica.

Authors:  C A Ison; C M Bellinger; J Walker
Journal:  J Clin Pathol       Date:  1986-10       Impact factor: 3.411

3.  Construction of isogenic gonococcal strains varying in the presence of a 4.2-kilobase cryptic plasmid.

Authors:  G D Biswas; J Graves; R Schwalbe; P F Sparling
Journal:  J Bacteriol       Date:  1986-08       Impact factor: 3.490

4.  Molecular characterization and comparison of plasmid content in seven different strains of Neisseria gonorrhoeae.

Authors:  R B Aalen; W B Gundersen
Journal:  Acta Pathol Microbiol Immunol Scand B       Date:  1987-02

5.  Polypeptides encoded by cryptic plasmids from Neisseria gonorrhoeae.

Authors:  R B Aalen; W B Gundersen
Journal:  Plasmid       Date:  1985-11       Impact factor: 3.466

Review 6.  Historical perspectives and identification of Neisseria and related species.

Authors:  J S Knapp
Journal:  Clin Microbiol Rev       Date:  1988-10       Impact factor: 26.132

7.  Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA.

Authors:  R Rossau; E Vanmechelen; J De Ley; H Van Heuverswijn
Journal:  J Gen Microbiol       Date:  1989-06

8.  Prevalence of gonococci lacking the 2.6 megadalton cryptic plasmid.

Authors:  D Abeck; F Alexander; A P Johnson; H C Korting; D Taylor-Robinson
Journal:  Lancet       Date:  1987-06-20       Impact factor: 79.321

9.  Evaluation of a prototype DNA probe test for the noncultural diagnosis of gonorrhea.

Authors:  P A Granato; M R Franz
Journal:  J Clin Microbiol       Date:  1989-04       Impact factor: 5.948

10.  Cryptic plasmid of Neisseria gonorrhoeae: complete nucleotide sequence and genetic organization.

Authors:  C Korch; P Hagblom; H Ohman; M Göransson; S Normark
Journal:  J Bacteriol       Date:  1985-08       Impact factor: 3.490
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  7 in total

1.  Evaluation of a fluorescent DNA hybridization assay for the detection of Neisseria gonorrhoeae.

Authors:  R J Cano; J C Palomares; M J Torres; R E Klem
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-07       Impact factor: 3.267

2.  Direct identification of bacterial isolates in blood cultures by using a DNA probe.

Authors:  T E Davis; D D Fuller
Journal:  J Clin Microbiol       Date:  1991-10       Impact factor: 5.948

3.  Polymerase chain reaction for the detection of Neisseria gonorrhoeae in clinical samples.

Authors:  B S Ho; W G Feng; B K Wong; S I Egglestone
Journal:  J Clin Pathol       Date:  1992-05       Impact factor: 3.411

Review 4.  Microbiological diagnosis of gonorrhoea.

Authors:  A E Jephcott
Journal:  Genitourin Med       Date:  1997-08

Review 5.  Laboratory methods in genitourinary medicine. Methods of diagnosing gonorrhoea.

Authors:  C A Ison
Journal:  Genitourin Med       Date:  1990-12

6.  Comparison of monoclonal antibody methods and a ribosomal ribonucleic acid probe test for Neisseria gonorrhoeae culture confirmation.

Authors:  W M Janda; L M Wilcoski; K L Mandel; P Ruther; J M Stevens
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1993-03       Impact factor: 3.267

7.  Evaluation of a urease-based confirmatory enzyme-linked immunosorbent assay for diagnosis of Neisseria gonorrhoeae.

Authors:  M Carballo; J R Dillon; M Lussier; P Milthorp; S Winston; B Brodeur
Journal:  J Clin Microbiol       Date:  1992-08       Impact factor: 5.948
  7 in total

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