Literature DB >> 2482333

Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA.

R Rossau1, E Vanmechelen, J De Ley, H Van Heuverswijn.   

Abstract

Eighteen sequences complementary to less-conserved regions within the 16S and 23S ribosomal ribonucleic acid (rRNA) of Neisseria gonorrhoeae were subcloned or chemically synthesized and used as probes in a dot-spot deoxyribonucleic acid (DNA): DNA hybridization format. Some of these probes exclusively detected Neisseria gonorrhoeae nucleic acid, whereas others also showed hybridization signals with nucleic acid from other bacterial species. Our results indicate that rRNA-derived DNA-probes can be used to differentiate between very closely related taxa without the use of Southern-blot analysis.

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Year:  1989        PMID： 2482333     DOI： 10.1099/00221287-135-6-1735

Source DB:  PubMed          Journal:  J Gen Microbiol        ISSN： 0022-1287


  11 in total

1.  Evaluation of a fluorescent DNA hybridization assay for the detection of Neisseria gonorrhoeae.

Authors:  R J Cano; J C Palomares; M J Torres; R E Klem
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1992-07       Impact factor: 3.267

2.  Polymerase chain reaction for the detection of Neisseria gonorrhoeae in clinical samples.

Authors:  B S Ho; W G Feng; B K Wong; S I Egglestone
Journal:  J Clin Pathol       Date:  1992-05       Impact factor: 3.411

3.  Simplified PCR for detection of Haemophilus ducreyi and diagnosis of chancroid.

Authors:  B West; S M Wilson; J Changalucha; S Patel; P Mayaud; R C Ballard; D Mabey
Journal:  J Clin Microbiol       Date:  1995-04       Impact factor: 5.948

4.  Antimicrobial susceptibility of Neisseria gonorrhoeae in Zaire: high level plasmid-mediated tetracycline resistance in central Africa.

Authors:  E Van Dyck; R Rossau; M Duhamel; F Behets; M Laga; M Nzila; S Bygdeman; H Van Heuverswijn; P Piot
Journal:  Genitourin Med       Date:  1992-04

5.  Elimination of contaminating DNA within polymerase chain reaction reagents: implications for a general approach to detection of uncultured pathogens.

Authors:  A Meier; D H Persing; M Finken; E C Böttger
Journal:  J Clin Microbiol       Date:  1993-03       Impact factor: 5.948

6.  Detection and identification of mycobacteria by amplification of rRNA.

Authors:  B Böddinghaus; T Rogall; T Flohr; H Blöcker; E C Böttger
Journal:  J Clin Microbiol       Date:  1990-08       Impact factor: 5.948

7.  Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae.

Authors:  R Rossau; M Duhamel; E Van Dyck; P Piot; H Van Heuverswyn
Journal:  J Clin Microbiol       Date:  1990-05       Impact factor: 5.948

8.  Identification of Bacteroides forsythus in subgingival plaque from patients with advanced periodontitis.

Authors:  H Gersdorf; A Meissner; K Pelz; G Krekeler; U B Göbel
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

9.  Comparison of monoclonal antibody methods and a ribosomal ribonucleic acid probe test for Neisseria gonorrhoeae culture confirmation.

Authors:  W M Janda; L M Wilcoski; K L Mandel; P Ruther; J M Stevens
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1993-03       Impact factor: 3.267

10.  Isolation and characterization of a species-specific DNA probe for Candida albicans.

Authors:  I Oren; E K Manavathu; S A Lerner
Journal:  Nucleic Acids Res       Date:  1991-12       Impact factor: 16.971
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