Literature DB >> 1500530

Evaluation of a urease-based confirmatory enzyme-linked immunosorbent assay for diagnosis of Neisseria gonorrhoeae.

M Carballo1, J R Dillon, M Lussier, P Milthorp, S Winston, B Brodeur.   

Abstract

A new urease-based enzyme-linked immunosorbent assay utilizing novel monoclonal antibodies was evaluated for the culture confirmation of Neisseria gonorrhoeae, with 270 isolates of N. gonorrhoeae, 56 isolates of diverse Neisseria spp., and 29 Moraxella isolates. The test was highly specific (100.00%) and sensitive (97.83%). No cross-reactions were observed with any of the Neisseria or Moraxella isolates tested. Fifty percent (3 of 6) of the false-negative results were obtained with isolates of serovar IA-4, a serovar rarely encountered in North America.

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Year:  1992        PMID: 1500530      PMCID: PMC265467          DOI: 10.1128/jcm.30.8.2181-2183.1992

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  23 in total

1.  A Simple Medium for Identification and Maintenance of the Gonococcus and Other Bacteria.

Authors:  H D Vera
Journal:  J Bacteriol       Date:  1948-04       Impact factor: 3.490

Review 2.  Serotyping Neisseria gonorrhoeae: a report of the Fourth International Workshop.

Authors:  M J Gill
Journal:  Genitourin Med       Date:  1991-02

3.  Evaluation of five rapid systems for the identification of Neisseria gonorrhoeae.

Authors:  J Dolter; L Bryant; J M Janda
Journal:  Diagn Microbiol Infect Dis       Date:  1990 May-Jun       Impact factor: 2.803

4.  Monoclonal hybridoma antibodies against human IgE and their use in a rapid and sensitive enzyme immunoassay for the semiquantitative assessment of total IgE levels in human blood.

Authors:  H M Chandler; A R Coulter; K Healey; M Kornitschuk; A MacGregor; J G Hurrell
Journal:  Int Arch Allergy Appl Immunol       Date:  1983

5.  Identification of Neisseria gonorrhoeae with the ORTHOProbe DNA probe test.

Authors:  A P Kuritza; S C Edberg; C Chapis; P Gallo
Journal:  Diagn Microbiol Infect Dis       Date:  1989 Mar-Apr       Impact factor: 2.803

6.  Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae.

Authors:  R Rossau; M Duhamel; E Van Dyck; P Piot; H Van Heuverswyn
Journal:  J Clin Microbiol       Date:  1990-05       Impact factor: 5.948

7.  Enzyme-linked immunosorbent assays for the detection of Neisseria gonorrhoeae specific antibodies.

Authors:  B R Brodeur; F E Ashton; B B Diena
Journal:  Can J Microbiol       Date:  1978-11       Impact factor: 2.419

8.  Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae.

Authors:  D C Yong; A Prytula
Journal:  J Clin Microbiol       Date:  1978-12       Impact factor: 5.948

9.  Validity of an enzyme immunoassay for detection of Neisseria gonorrhoeae antigens.

Authors:  C J Papasian; W R Bartholomew; D Amsterdam
Journal:  J Clin Microbiol       Date:  1984-03       Impact factor: 5.948

10.  Evaluation of a fluorescent monoclonal antibody reagent for identification of cultured Neisseria gonorrhoeae.

Authors:  C A Ison; A Tanna; C S Easmon
Journal:  J Med Microbiol       Date:  1988-06       Impact factor: 2.472

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  1 in total

1.  Neisseria flavescens: A Urease-Expressing Potential Pathogen Isolated from Gastritis Patients.

Authors:  Bo Zeng; Lijin Sun; Yongmei Chen; Yin Qian; Qian Cao; Zhi Zhang; Zaixin Li
Journal:  Curr Microbiol       Date:  2017-10-24       Impact factor: 2.188

  1 in total

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