D Liu1, E Swiatlo, F W Austin, M L Lawrence. 1. College of Veterinary Medicine, Mississippi State University, Mississippi State, MS 39762, USA. liu@cvm.msstate.edu
Abstract
AIMS: To investigate the use of a Staphylococcus epidermidis transcriptional regulator gene as target for species-specific determination. METHODS AND RESULTS: Staph. epidermidis genes encoding putative transcriptional regulators were retrieved from GenBank and those showing no homology with other bacterial sequences were selected. Of the four PCR primer sets analysed, the primers Serp0107F/R from serp0107 amplified a specific product of 581 bp from Staph. epidermidis DNA only, and they did not cross-react in PCR with nonepidermidis staphylococci and other common bacteria. CONCLUSION: Being uniquely present in Staph. epidermidis, putative transcriptional regulator gene serp0107 offers a valuable target for specific identification of Staph. epidermidis. SIGNIFICANCE AND IMPACT OF THE STUDY: As a member of a specialized gene group, putative transcriptional regulator gene serp0107 may be important to Staph. epidermidis adaptation to its niche environment. Further analysis of serp0107 and its related protein may help reveal new insights on the molecular regulation of Staph. epidermidis survival and virulence.
AIMS: To investigate the use of a Staphylococcus epidermidis transcriptional regulator gene as target for species-specific determination. METHODS AND RESULTS: Staph. epidermidis genes encoding putative transcriptional regulators were retrieved from GenBank and those showing no homology with other bacterial sequences were selected. Of the four PCR primer sets analysed, the primers Serp0107F/R from serp0107 amplified a specific product of 581 bp from Staph. epidermidis DNA only, and they did not cross-react in PCR with nonepidermidis staphylococci and other common bacteria. CONCLUSION: Being uniquely present in Staph. epidermidis, putative transcriptional regulator gene serp0107 offers a valuable target for specific identification of Staph. epidermidis. SIGNIFICANCE AND IMPACT OF THE STUDY: As a member of a specialized gene group, putative transcriptional regulator gene serp0107 may be important to Staph. epidermidis adaptation to its niche environment. Further analysis of serp0107 and its related protein may help reveal new insights on the molecular regulation of Staph. epidermidis survival and virulence.
Authors: Florence Tchouaffi-Nana; T Eric Ballard; Christine H Cary; Timothy L Macdonald; Costi D Sifri; Paul S Hoffman Journal: Antimicrob Agents Chemother Date: 2010-04-19 Impact factor: 5.191
Authors: Young Kyung Park; Young Hwan Paik; Jang Won Yoon; Lawrence K Fox; Sun Young Hwang; Yong Ho Park Journal: J Vet Sci Date: 2013-06-30 Impact factor: 1.672