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Literature DB >> 1690391

Infectious DNA of the human spumaretrovirus.

A Rethwilm¹, G Baunach, K O Netzer, B Maurer, B Borisch, V ter Meulen.

Abstract

An infectious molecular clone (pHSRV) of the human Spumaretrovirus (HSRV) was constructed using viral DNA and cDNA clones. The infectivity of pHSRV was proven by transfection of cell cultures and subsequent infection of susceptible cultures with cell free transfection derived virus. pHSRV derived virus produced foamy virus typical cytopathic effects in susceptible cultures. Infected cells could be stained specifically with foamy virus antisera by means of indirect immunofluorescence. Radioimmunoprecipitation revealed the presence of characteristic HSRV structural proteins in pHSRV infected cultures. By cotransfection of pHSRV and an indicator plasmid it was found that pHSRV is able to transactivate the viral LTR. Viral transcripts were found to be approximately 200 bases longer in pHSRV infected cultures compared to wildtype infected cultures. This difference is most likely due to an insertion of DNA of non-viral origin in the U3 region of the 3'LTR of the infectious clone.

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Year: 1990 PMID： 1690391 PMCID： PMC330320 DOI： 10.1093/nar/18.4.733

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

21 in total

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Authors: B G Achong; P W Mansell; M A Epstein; P Clifford
Journal: J Natl Cancer Inst Date: 1971-02 Impact factor: 13.506

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Authors: G Henle; W Henle
Journal: J Bacteriol Date: 1966-03 Impact factor: 3.490

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Authors: D Young; J Samuels; J K Clarke
Journal: Arch Gesamte Virusforsch Date: 1973

4. A new technique for the assay of infectivity of human adenovirus 5 DNA.

Authors: F L Graham; A J van der Eb
Journal: Virology Date: 1973-04 Impact factor: 3.616

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Authors: K R Cameron; S M Birchall; M A Moses
Journal: Lancet Date: 1978-10-07 Impact factor: 79.321

6. Isolation of foamy virus from patients with de Quervain thyroiditis.

Authors: J Werner; H Gelderblom
Journal: Lancet Date: 1979-08-04 Impact factor: 79.321

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Authors: A P Feinberg; B Vogelstein
Journal: Anal Biochem Date: 1984-02 Impact factor: 3.365

8. Isolation and some serological and epidemiological data on the viruses recovered from patients with subacute thyroiditis de Quervain.

Authors: D Stancek; M Stanceková-Gressnerová; M Janotka; P Hnilica; D Oravec
Journal: Med Microbiol Immunol Date: 1975 Impact factor: 3.402

9. Characterization of a foamy virus isolated from Cercopithecus aethiops lymphoblastoid cells.

Authors: D Neumann-Haefelin; A Rethwilm; G Bauer; F Gudat; H zur Hausen
Journal: Med Microbiol Immunol Date: 1983 Impact factor: 3.402

10. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Authors: J M Chirgwin; A E Przybyla; R J MacDonald; W J Rutter
Journal: Biochemistry Date: 1979-11-27 Impact factor: 3.162

27 in total

Review 1. Mechanism of action of regulatory proteins encoded by complex retroviruses.

Authors: B R Cullen
Journal: Microbiol Rev Date: 1992-09

2. Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro.

Authors: A Engelman; R Craigie
Journal: J Virol Date: 1992-11 Impact factor: 5.103

3. Distinct cis-acting regions in U3 regulate trans-activation of the human spumaretrovirus long terminal repeat by the viral bel1 gene product.

Authors: L K Venkatesh; P A Theodorakis; G Chinnadurai
Journal: Nucleic Acids Res Date: 1991-07-11 Impact factor: 16.971

Infectious DNA of the human spumaretrovirus.

1. An unusual virus in cultures from a human nasopharyngeal carcinoma.

2. Immunofluorescence in cells derived from Burkitt's lymphoma.

3. A foamy virus of possible human origin isolated in BHK-21 cells.

4. A new technique for the assay of infectivity of human adenovirus 5 DNA.

5. Isolation of foamy virus from patient with dialysis encephalopathy.

6. Isolation of foamy virus from patients with de Quervain thyroiditis.

7. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.

8. Isolation and some serological and epidemiological data on the viruses recovered from patients with subacute thyroiditis de Quervain.

9. Characterization of a foamy virus isolated from Cercopithecus aethiops lymphoblastoid cells.

10. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.

Review 1. Mechanism of action of regulatory proteins encoded by complex retroviruses.

2. Identification of conserved amino acid residues critical for human immunodeficiency virus type 1 integrase function in vitro.

3. Distinct cis-acting regions in U3 regulate trans-activation of the human spumaretrovirus long terminal repeat by the viral bel1 gene product.

4. Analysis of splicing patterns of human spumaretrovirus by polymerase chain reaction reveals complex RNA structures.

5. Characterization of the transcriptional trans activator of human foamy retrovirus.

Review 6. Foamy viruses are unconventional retroviruses.

7. Characterization of the R572T point mutant of a putative cleavage site in human foamy virus Env.

8. The transcriptional transactivator of human foamy virus maps to the bel 1 genomic region.

Review 9. Transgenic and knockout mice in the study of neurodegenerative diseases.

10. Functional analysis of human foamy virus accessory reading frames.