Literature DB >> 16847258

A global topology map of the Saccharomyces cerevisiae membrane proteome.

Hyun Kim1, Karin Melén, Marie Osterberg, Gunnar von Heijne.   

Abstract

The yeast Saccharomyces cerevisiae is, arguably, the best understood eukaryotic model organism, yet comparatively little is known about its membrane proteome. Here, we report the cloning and expression of 617 S. cerevisiae membrane proteins as fusions to a C-terminal topology reporter and present experimentally constrained topology models for 546 proteins. By homology, the experimental topology information can be extended to approximately 15,000 membrane proteins from 38 fully sequenced eukaryotic genomes.

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Year:  2006        PMID: 16847258      PMCID: PMC1544055          DOI: 10.1073/pnas.0604075103

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN: 0027-8424            Impact factor:   11.205


  57 in total

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Authors:  A Krogh; B Larsson; G von Heijne; E L Sonnhammer
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Authors:  A Sääf; M Johansson; E Wallin; G von Heijne
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Authors:  J D Romano; S Michaelis
Journal:  Mol Biol Cell       Date:  2001-07       Impact factor: 4.138

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Review 8.  Life with 6000 genes.

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10.  The distribution of positively charged residues in bacterial inner membrane proteins correlates with the trans-membrane topology.

Authors:  G Heijne
Journal:  EMBO J       Date:  1986-11       Impact factor: 11.598

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