| Literature DB >> 16847258 |
Hyun Kim1, Karin Melén, Marie Osterberg, Gunnar von Heijne.
Abstract
The yeast Saccharomyces cerevisiae is, arguably, the best understood eukaryotic model organism, yet comparatively little is known about its membrane proteome. Here, we report the cloning and expression of 617 S. cerevisiae membrane proteins as fusions to a C-terminal topology reporter and present experimentally constrained topology models for 546 proteins. By homology, the experimental topology information can be extended to approximately 15,000 membrane proteins from 38 fully sequenced eukaryotic genomes.Entities:
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Year: 2006 PMID: 16847258 PMCID: PMC1544055 DOI: 10.1073/pnas.0604075103
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN: 0027-8424 Impact factor: 11.205