| Literature DB >> 16843591 |
Pedro Casado1, Pedro Zuazua-Villar, Eva del Valle, Carlos Martínez-Campa, Pedro S Lazo, Sofía Ramos.
Abstract
Vincristine is an antitumor drug that inhibits microtubule polymerization, causes G2/M arrest and induces apoptosis. 2D-PAGE and MALDI-TOF-MS analysis of vincristine effects on MCF7 cells, revealed a vincristine upregulated form and a vincristine downregulated form of the antiapoptotic protein HSP27. These findings linked to the lack of vincristine effect over HSP27 mRNA, suggest a protein post-translational modification. Further assays indicated the presence of a phosphorylated peptide, containing serine 82, only in the vincristine upregulated form. Serine 82 phosphorylation was confirmed using specific antibodies. Thus, phosphorylation of HSP27 may play a role in the cellular response to vincristine.Entities:
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Year: 2006 PMID： 16843591 DOI： 10.1016/j.canlet.2006.05.005
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679