Literature DB >> 16825394

Identification of bacteria in formalin-fixed, paraffin-embedded heart valve tissue via 16S rRNA gene nucleotide sequencing.

Kavita Imrit1, Michael Goldfischer, Jie Wang, Jaime Green, Jerome Levine, Joseph Lombardo, Tao Hong.   

Abstract

We applied 16S rRNA gene sequencing to identify bacterial species present in formalin-fixed, paraffin-embedded heart valve tissue. In 40% (12/30) of the cases, we were able to identify the bacterium to the species-genus level. For more recent cases (<or=4 years), the success rate was significantly improved, to 70% (P<0.001).

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Year:  2006        PMID: 16825394      PMCID: PMC1489487          DOI: 10.1128/JCM.00572-06

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  14 in total

1.  Nonculture Laboratory Methods for the Diagnosis of Infectious Endocarditis.

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Journal:  Curr Infect Dis Rep       Date:  1999-06       Impact factor: 3.725

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Authors:  Clarisse Rovery; Gilbert Greub; Hubert Lepidi; Jean-Paul Casalta; Gilbert Habib; Frédéric Collart; Didier Raoult
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Review 3.  Sequence-based identification of new bacteria: a proposition for creation of an orphan bacterium repository.

Authors:  M Drancourt; D Raoult
Journal:  J Clin Microbiol       Date:  2005-09       Impact factor: 5.948

Review 4.  Infective endocarditis due to unusual or fastidious microorganisms.

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Journal:  Mayo Clin Proc       Date:  1997-06       Impact factor: 7.616

Review 5.  Destructive endocarditis caused by Staphylococcus lugdunensis. Case report and review of the literature.

Authors:  G De Hondt; M Ieven; C Vandermersch; J Colaert
Journal:  Acta Clin Belg       Date:  1997       Impact factor: 1.264

6.  Molecular diagnosis of culture negative infective endocarditis: clinical validation in a group of surgically treated patients.

Authors:  M Grijalva; R Horváth; M Dendis; J Erný; J Benedík
Journal:  Heart       Date:  2003-03       Impact factor: 5.994

7.  Infective endocarditis in patients with negative blood cultures: analysis of 88 cases from a one-year nationwide survey in France.

Authors:  B Hoen; C Selton-Suty; F Lacassin; J Etienne; S Briançon; C Leport; P Canton
Journal:  Clin Infect Dis       Date:  1995-03       Impact factor: 9.079

Review 8.  Diagnostic methods current best practices and guidelines for histologic evaluation in infective endocarditis.

Authors:  Hubert Lepidi; David T Durack; Didier Raoult
Journal:  Infect Dis Clin North Am       Date:  2002-06       Impact factor: 5.982

9.  New criteria for diagnosis of infective endocarditis: utilization of specific echocardiographic findings. Duke Endocarditis Service.

Authors:  D T Durack; A S Lukes; D K Bright
Journal:  Am J Med       Date:  1994-03       Impact factor: 4.965

Review 10.  Endocarditis due to Staphylococcus lugdunensis: report of 11 cases and review.

Authors:  F Vandenesch; J Etienne; M E Reverdy; S J Eykyn
Journal:  Clin Infect Dis       Date:  1993-11       Impact factor: 9.079

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3.  PCR-electrospray ionization mass spectrometry for direct detection of pathogens and antimicrobial resistance from heart valves in patients with infective endocarditis.

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Review 5.  Characterization of the gut microbiome in epidemiologic studies: the multiethnic cohort experience.

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7.  Duplex PCR assay simultaneously detecting and differentiating Bartonella quintana, B. henselae, and Coxiella burnetii in surgical heart valve specimens.

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8.  Clinical, epidemiologic, histopathologic and molecular features of an unexplained dermopathy.

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10.  Stereomicroscopic 3D-pattern profiling of murine and human intestinal inflammation reveals unique structural phenotypes.

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