Literature DB >> 19553582

Duplex PCR assay simultaneously detecting and differentiating Bartonella quintana, B. henselae, and Coxiella burnetii in surgical heart valve specimens.

Yi-Wei Tang1.   

Abstract

A duplex PCR (dPCR) assay was developed to simultaneously detect and differentiate Bartonella quintana, Bartonella henselae, and Coxiella burnetii from surgical heart valve tissue specimens with an analytic sensitivity of 10 copies/reaction. Among 17 specimens collected from patients with a clinical diagnosis of culture-negative endocarditis, 2, 4, and 2 were positive for B. quintana, B. henselae, and C. burnetii, respectively, by the dPCR assay, which matched the results obtained by universal bacterial 16S rRNA gene amplification and sequencing.

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Year:  2009        PMID： 19553582      PMCID： PMC2725655          DOI： 10.1128/JCM.00721-09

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  24 in total

1.  Identification of the bacterial etiology of culture-negative endocarditis by amplification and sequencing of a small ribosomal RNA gene.

Authors:  Irakli Khulordava; Geraldine Miller; David Haas; Haijing Li; Joel McKinsey; Daniel Vanderende; Yi-Wei Tang
Journal:  Diagn Microbiol Infect Dis       Date:  2003-05       Impact factor: 2.803

2.  A Coxiella burnetti repeated DNA element resembling a bacterial insertion sequence.

Authors:  T A Hoover; M H Vodkin; J C Williams
Journal:  J Bacteriol       Date:  1992-09       Impact factor: 3.490

3.  Identification and elimination of DNA sequences in Taq DNA polymerase.

Authors:  M S Hughes; L A Beck; R A Skuce
Journal:  J Clin Microbiol       Date:  1994-08       Impact factor: 5.948

4.  Frequent contamination of Taq polymerase with DNA.

Authors:  E C Böttger
Journal:  Clin Chem       Date:  1990-06       Impact factor: 8.327

5.  Identification of coryneform bacterial isolates by ribosomal DNA sequence analysis.

Authors:  Y W Tang; A Von Graevenitz; M G Waddington; M K Hopkins; D H Smith; H Li; C P Kolbert; S O Montgomery; D H Persing
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

Review 6.  Endocarditis due to rare and fastidious bacteria.

Authors:  P Brouqui; D Raoult
Journal:  Clin Microbiol Rev       Date:  2001-01       Impact factor: 26.132

7.  Etiologic diagnosis of infective endocarditis by broad-range polymerase chain reaction: a 3-year experience.

Authors:  Philipp Peter Bosshard; Andreas Kronenberg; Reinhard Zbinden; Christian Ruef; Erik Christian Böttger; Martin Altwegg
Journal:  Clin Infect Dis       Date:  2003-07-09       Impact factor: 9.079

8.  Evaluation of PCR in the molecular diagnosis of endocarditis.

Authors:  S Lang; R W Watkin; P A Lambert; R S Bonser; W A Littler; T S J Elliott
Journal:  J Infect       Date:  2004-04       Impact factor: 6.072

9.  Outcome and treatment of Bartonella endocarditis.

Authors:  Didier Raoult; Pierre-Edouard Fournier; François Vandenesch; Jean-Luc Mainardi; Susannah J Eykyn; James Nash; Edward James; Catherine Benoit-Lemercier; Thomas J Marrie
Journal:  Arch Intern Med       Date:  2003-01-27

10.  Measurement of human cytomegalovirus loads by quantitative real-time PCR for monitoring clinical intervention in transplant recipients.

Authors:  Haijing Li; J Stephen Dummer; Wray R Estes; Shufang Meng; Peter F Wright; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2003-01       Impact factor: 5.948
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  6 in total

1.  Mycobacterium smegmatis in skin biopsy specimens from patients with suppurative granulomatous inflammation.

Authors:  Zhe Xu; Di Lu; Xia Zhang; Haijing Li; Shufang Meng; Yue-Song Pan; Alan S Boyd; Lin Ma; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2013-01-09       Impact factor: 5.948

2.  Absence of gastrointestinal pathogens in ileum tissue resected for necrotizing enterocolitis.

Authors:  Tim Ullrich; Yi-Wei Tang; Hernan Correa; Steven A Garzon; Akhil Maheshwari; Melissa Hill; Pranathi Matta; Mohan K Krishnan; Jörn-Hendrik Weitkamp
Journal:  Pediatr Infect Dis J       Date:  2012-04       Impact factor: 2.129

3.  PCR-electrospray ionization mass spectrometry for direct detection of pathogens and antimicrobial resistance from heart valves in patients with infective endocarditis.

Authors:  Cassandra L Brinkman; Paschalis Vergidis; James R Uhl; Bobbi S Pritt; Franklin R Cockerill; James M Steckelberg; Larry M Baddour; Joseph J Maleszewski; William D Edwards; Rangarajan Sampath; Robin Patel
Journal:  J Clin Microbiol       Date:  2013-04-17       Impact factor: 5.948

4.  Bartonella henselae aortic valve endocarditis mimicking systemic vasculitis.

Authors:  Laurence S G Teoh; Hamish H Hart; May Ching Soh; Jonathan P Christiansen; Hasan Bhally; Martin S Philips; Dominic S Rai-Chaudhuri
Journal:  BMJ Case Rep       Date:  2010-10-21

Review 5.  Molecular methods for pathogen and microbial community detection and characterization: current and potential application in diagnostic microbiology.

Authors:  Christopher D Sibley; Gisele Peirano; Deirdre L Church
Journal:  Infect Genet Evol       Date:  2012-02-09       Impact factor: 3.342

6.  Association of Bartonella spp bacteremia with Chagas cardiomyopathy, endocarditis and arrhythmias in patients from South America.

Authors:  F G Corrêa; C L S Pontes; R M M Verzola; J C P Mateos; P E N F Velho; A G Schijman; H S Selistre-de-Araujo
Journal:  Braz J Med Biol Res       Date:  2012-05-17       Impact factor: 2.590
  6 in total

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