| Literature DB >> 16820683 |
Zhongli Cui1, Yukie Maruyama, Bunzo Mikami, Wataru Hashimoto, Kousaku Murata.
Abstract
Alpha-L-rhamnosidases play important roles in the metabolism of plant cell walls, glycosides and bacterial biofilms. This enzyme is also used industrially for debittering citrus fruits by releasing rhamnose from the plant flavonoid naringin. Bacillus sp. GL1 alpha-L-rhamnosidase (RhaB) is a member of glycoside hydrolase (GH) family 78. Native and selenomethionine-derivative enzymes were crystallized at 293 K by hanging-drop vapour diffusion with polyethylene glycol 8000 as a precipitant. This is the first report of the crystallization of a family GH78 enzyme.Entities:
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Year: 2006 PMID： 16820683 PMCID： PMC2242939 DOI： 10.1107/S174430910601904X
Source DB: PubMed Journal: Acta Crystallogr Sect F Struct Biol Cryst Commun ISSN： 1744-3091