Literature DB >> 16809540

Structural basis of RNA-dependent recruitment of glutamine to the genetic code.

Hiroyuki Oshikane1, Kelly Sheppard, Shuya Fukai, Yuko Nakamura, Ryuichiro Ishitani, Tomoyuki Numata, R Lynn Sherrer, Liang Feng, Emmanuelle Schmitt, Michel Panvert, Sylvain Blanquet, Yves Mechulam, Dieter Söll, Osamu Nureki.   

Abstract

Glutaminyl-transfer RNA (Gln-tRNA(Gln)) in archaea is synthesized in a pretranslational amidation of misacylated Glu-tRNA(Gln) by the heterodimeric Glu-tRNA(Gln) amidotransferase GatDE. Here we report the crystal structure of the Methanothermobacter thermautotrophicus GatDE complexed to tRNA(Gln) at 3.15 angstroms resolution. Biochemical analysis of GatDE and of tRNA(Gln) mutants characterized the catalytic centers for the enzyme's three reactions (glutaminase, kinase, and amidotransferase activity). A 40 angstrom-long channel for ammonia transport connects the active sites in GatD and GatE. tRNA(Gln) recognition by indirect readout based on shape complementarity of the D loop suggests an early anticodon-independent RNA-based mechanism for adding glutamine to the genetic code.

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Year:  2006        PMID： 16809540     DOI： 10.1126/science.1128470

Source DB:  PubMed          Journal:  Science        ISSN： 0036-8075            Impact factor:   47.728


  43 in total

1.  Co-evolution of the archaeal tRNA-dependent amidotransferase GatCAB with tRNA(Asn).

Authors:  Suk Namgoong; Kelly Sheppard; R Lynn Sherrer; Dieter Söll
Journal:  FEBS Lett       Date:  2007-01-02       Impact factor: 4.124

2.  Novel multiprotein complexes identified in the hyperthermophilic archaeon Pyrococcus furiosus by non-denaturing fractionation of the native proteome.

Authors:  Angeli Lal Menon; Farris L Poole; Aleksandar Cvetkovic; Sunia A Trauger; Ewa Kalisiak; Joseph W Scott; Saratchandra Shanmukh; Jeremy Praissman; Francis E Jenney; William R Wikoff; John V Apon; Gary Siuzdak; Michael W W Adams
Journal:  Mol Cell Proteomics       Date:  2008-11-28       Impact factor: 5.911

Review 3.  Aminoacyl-tRNA synthetase complexes: molecular multitasking revealed.

Authors:  Corinne D Hausmann; Michael Ibba
Journal:  FEMS Microbiol Rev       Date:  2008-06-03       Impact factor: 16.408

4.  Two-step aminoacylation of tRNA without channeling in Archaea.

Authors:  Hari Bhaskaran; John J Perona
Journal:  J Mol Biol       Date:  2011-06-25       Impact factor: 5.469

Review 5.  tRNAs: cellular barcodes for amino acids.

Authors:  Rajat Banerjee; Shawn Chen; Kiley Dare; Marla Gilreath; Mette Praetorius-Ibba; Medha Raina; Noah M Reynolds; Theresa Rogers; Hervé Roy; Srujana S Yadavalli; Michael Ibba
Journal:  FEBS Lett       Date:  2010-01-21       Impact factor: 4.124

6.  The archaeal transamidosome for RNA-dependent glutamine biosynthesis.

Authors:  Theodoros Rampias; Kelly Sheppard; Dieter Söll
Journal:  Nucleic Acids Res       Date:  2010-05-10       Impact factor: 16.971

7.  Methanothermobacter thermautotrophicus tRNA Gln confines the amidotransferase GatCAB to asparaginyl-tRNA Asn formation.

Authors:  Kelly Sheppard; R Lynn Sherrer; Dieter Söll
Journal:  J Mol Biol       Date:  2008-01-31       Impact factor: 5.469

8.  On the evolution of the tRNA-dependent amidotransferases, GatCAB and GatDE.

Authors:  Kelly Sheppard; Dieter Söll
Journal:  J Mol Biol       Date:  2008-01-16       Impact factor: 5.469

9.  Recognition of tRNAGln by Helicobacter pylori GluRS2--a tRNAGln-specific glutamyl-tRNA synthetase.

Authors:  Keng-Ming Chang; Tamara L Hendrickson
Journal:  Nucleic Acids Res       Date:  2009-09-15       Impact factor: 16.971

10.  Two distinct regions in Staphylococcus aureus GatCAB guarantee accurate tRNA recognition.

Authors:  Akiyoshi Nakamura; Kelly Sheppard; Junji Yamane; Min Yao; Dieter Söll; Isao Tanaka
Journal:  Nucleic Acids Res       Date:  2009-11-11       Impact factor: 16.971
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