| Literature DB >> 16793515 |
Xin-Ying Jia1, Zhen-Hua Tian, Lei Shao, Xu-Dong Qu, Qun-Fei Zhao, Jian Tang, Gong-Li Tang, Wen Liu.
Abstract
The biosynthetic gene cluster for chlorothricin (CHL) was localized to a 122 kb contiguous DNA from Streptomyces antibioticus DSM 40725, and its involvement in CHL biosynthesis was confirmed by gene inactivation and complementation. Bioinformatic analysis of the sequenced 111.989 kb DNA region revealed 42 open reading frames, 35 of which were defined to constitute the CHL gene cluster. An assembly model for CHL biosynthesis from D-olivose, 2-methoxy-5-chloro-6-methylsalicyclic acid, and chlorothricolide building blocks was proposed. This work represents cloning of a gene cluster for spirotetronate antibiotic biosynthesis and sets the stage to investigate the unusual macrolide biosynthesis including tandem Diels-Alder cyclizations, Baeyer-Villiger oxidation, and incorporation of an enoylpyruvate unit.Entities:
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Year: 2006 PMID： 16793515 DOI： 10.1016/j.chembiol.2006.03.008
Source DB: PubMed Journal: Chem Biol ISSN： 1074-5521