Literature DB >> 16793394

Human DNA ligases I, III, and IV-purification and new specific assays for these enzymes.

Xi Chen1, John Pascal, Sangeetha Vijayakumar, Gerald M Wilson, Tom Ellenberger, Alan E Tomkinson.   

Abstract

The joining of DNA strand breaks by DNA ligases is required to seal Okazaki fragments during DNA replication and to complete almost all DNA repair pathways. In human cells, there are multiple species of DNA ligase encoded by the LIG1, LIG3, and LIG4 genes. Here we describe protocols to overexpress and purify recombinant DNA ligase I, DNA ligase IIIbeta, and DNA ligase IV/XRCC4 and the assays used to purify and distinguish between these enzymes. In addition, we describe a fluorescence-based ligation assay that can be used for high throughput screening of chemical libraries.

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Year:  2006        PMID： 16793394     DOI： 10.1016/S0076-6879(05)09003-8

Source DB:  PubMed          Journal:  Methods Enzymol        ISSN： 0076-6879            Impact factor:   1.600


  22 in total

1.  Substrate channeling in mammalian base excision repair pathways: passing the baton.

Authors:  Rajendra Prasad; David D Shock; William A Beard; Samuel H Wilson
Journal:  J Biol Chem       Date:  2010-10-14       Impact factor: 5.157

2.  Rational design of human DNA ligase inhibitors that target cellular DNA replication and repair.

Authors:  Xi Chen; Shijun Zhong; Xiao Zhu; Barbara Dziegielewska; Tom Ellenberger; Gerald M Wilson; Alexander D MacKerell; Alan E Tomkinson
Journal:  Cancer Res       Date:  2008-05-01       Impact factor: 12.701

3.  SCR7 is neither a selective nor a potent inhibitor of human DNA ligase IV.

Authors:  George E Greco; Yoshihiro Matsumoto; Rhys C Brooks; Zhengfei Lu; Michael R Lieber; Alan E Tomkinson
Journal:  DNA Repair (Amst)       Date:  2016-05-07

4.  A high-throughput scintillation proximity-based assay for human DNA ligase IV.

Authors:  Hui-Min Tseng; David Shum; Bhavneet Bhinder; Sindy Escobar; Nicholas J Veomett; Alan E Tomkinson; David Y Gin; Hakim Djaballah; David A Scheinberg
Journal:  Assay Drug Dev Technol       Date:  2011-12-22       Impact factor: 1.738

5.  Electron microscopy visualization of DNA-protein complexes formed by Ku and DNA ligase IV.

Authors:  Patricia Grob; Teri T Zhang; Ryan Hannah; Hui Yang; Melissa L Hefferin; Alan E Tomkinson; Eva Nogales
Journal:  DNA Repair (Amst)       Date:  2011-11-15

Review 6.  Non-homologous DNA end joining and alternative pathways to double-strand break repair.

Authors:  Howard H Y Chang; Nicholas R Pannunzio; Noritaka Adachi; Michael R Lieber
Journal:  Nat Rev Mol Cell Biol       Date:  2017-05-17       Impact factor: 94.444

7.  Role of polymerase β in complementing aprataxin deficiency during abasic-site base excision repair.

Authors:  Melike Cağlayan; Vinod K Batra; Akira Sassa; Rajendra Prasad; Samuel H Wilson
Journal:  Nat Struct Mol Biol       Date:  2014-04-28       Impact factor: 15.369

8.  CTG/CAG repeat instability is modulated by the levels of human DNA ligase I and its interaction with proliferating cell nuclear antigen: a distinction between replication and slipped-DNA repair.

Authors:  Arturo López Castel; Alan E Tomkinson; Christopher E Pearson
Journal:  J Biol Chem       Date:  2009-07-22       Impact factor: 5.157

9.  Phosphorylation of human DNA ligase I regulates its interaction with replication factor C and its participation in DNA replication and DNA repair.

Authors:  Sangeetha Vijayakumar; Barbara Dziegielewska; David S Levin; Wei Song; Jinhu Yin; Austin Yang; Yoshihiro Matsumoto; Vladimir P Bermudez; Jerard Hurwitz; Alan E Tomkinson
Journal:  Mol Cell Biol       Date:  2009-02-17       Impact factor: 4.272

10.  DNA sequence context effects on the glycosylase activity of human 8-oxoguanine DNA glycosylase.

Authors:  Akira Sassa; William A Beard; Rajendra Prasad; Samuel H Wilson
Journal:  J Biol Chem       Date:  2012-09-18       Impact factor: 5.157
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