Literature DB >> 16789217

Antibodies Against the Genome-Linked Protein VPg of Cowpea Mosaic Virus Recognize a 60,000-Dalton Precursor Polypeptide.

P Zabel1, M Moerman, F van Straaten, R Goldbach, A van Kammen.   

Abstract

We have prepared a rabbit antiserum specifically directed against the genome-linked protein (VPg) of cowpea mosaic virus by injecting an hydrolysate of purified virion RNA. Using this antiserum as a probe in combination with "Western" (protein) blots of subcellular fractions of cowpea mosaic virus-infected cowpea (Vigna unguiculata) cells, we have detected a bottom component RNA-encoded, 60,000-dalton polypeptide which is membrane bound and presumably represents the immediate precursor of VPg.

Entities:  

Year:  1982        PMID： 16789217      PMCID： PMC256847     

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  32 in total

1.  Protein bound to the genome RNAs of cowpea mosaic virus.

Authors:  S D Daubert; G Bruening; R C Najarian
Journal:  Eur J Biochem       Date:  1978-12-01

2.  The genome-linked protein of picornaviruses. IV. Difference in the VPg's of encephalomyocarditis virus and poliovirus as evidence that the genome-linked proteins are virus-coded.

Authors:  F Golini; A Nomoto; E Wimmer
Journal:  Virology       Date:  1978-08       Impact factor: 3.616

3.  Identification of a protein linked to nascent poliovirus RNA and to the polyuridylic acid of negative-strand RNA.

Authors:  R F Pettersson; V Ambros; D Baltimore
Journal:  J Virol       Date:  1978-08       Impact factor: 5.103

4.  Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.

Authors:  J B Flanegan; R F Petterson; V Ambros; N J Hewlett; D Baltimore
Journal:  Proc Natl Acad Sci U S A       Date:  1977-03       Impact factor: 11.205

5.  The location of the polio genome protein in viral RNAs and its implication for RNA synthesis.

Authors:  A Nomoto; B Detjen; R Pozzatti; E Wimmer
Journal:  Nature       Date:  1977-07-21       Impact factor: 49.962

6.  Protein covalently linked to foot-and-mouth disease virus RNA.

Authors:  D V Sangar; D J Rowlands; T J Harris; F Brown
Journal:  Nature       Date:  1977-08-18       Impact factor: 49.962

7.  Encephalomyocarditis virus RNA. III. Presence of a genome-associated protein.

Authors:  D E Hruby; W K Roberts
Journal:  J Virol       Date:  1978-01       Impact factor: 5.103

8.  Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure.

Authors:  J Renart; J Reiser; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1979-07       Impact factor: 11.205

9.  A protein linked to the 5' termini of both RNA components of the cowpea mosaic virus genome.

Authors:  J Stanley; P Rottier; J W Davies; P Zabel; A Van Kammen
Journal:  Nucleic Acids Res       Date:  1978-12       Impact factor: 16.971

10.  A protein covalently linked to poliovirus genome RNA.

Authors:  Y F Lee; A Nomoto; B M Detjen; E Wimmer
Journal:  Proc Natl Acad Sci U S A       Date:  1977-01       Impact factor: 11.205
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  13 in total

1.  Genetic and molecular characterization of an Adh-1 null mutant in tomato.

Authors:  E Wisman; M Koornneef; T Chase; E Lifshytz; M S Ramanna; P Zabel
Journal:  Mol Gen Genet       Date:  1991-04

2.  Orientation of the cleavage map of the 200-kilodalton polypeptide encoded by the bottom-component RNA of cowpea mosaic virus.

Authors:  R Goldbach; G Rezelman
Journal:  J Virol       Date:  1983-05       Impact factor: 5.103

3.  Antibody to a synthetic nonapeptide corresponding to the NH2 terminus of poliovirus genome-linked protein VPg reacts with native VPg and inhibits in vitro replication of poliovirus RNA.

Authors:  C D Morrow; A Dasgupta
Journal:  J Virol       Date:  1983-11       Impact factor: 5.103

4.  Expression of the Bottom-Component RNA of Cowpea Mosaic Virus: Evidence that the 60-Kilodalton VPg Precursor Is Cleaved into Single VPg and a 58-Kilodalton Polypeptide.

Authors:  R Goldbach; G Rezelman; P Zabel; A van Kammen
Journal:  J Virol       Date:  1982-05       Impact factor: 5.103

5.  Evidence That the 32,000-Dalton Protein Encoded by Bottom-Component RNA of Cowpea Mosaic Virus is a Proteolytic Processing Enzyme.

Authors:  H Franssen; M Moerman; G Rezelman; R Goldbach
Journal:  J Virol       Date:  1984-04       Impact factor: 5.103

6.  Cowpea Mosaic Virus-Encoded Protease Does Not Recognize Primary Translation Products of M RNAs from Other Comoviruses.

Authors:  R Goldbach; J Krijt
Journal:  J Virol       Date:  1982-09       Impact factor: 5.103

7.  Detection of a Novel Protein Encoded by the Bottom-Component RNA of Cowpea Mosaic Virus, Using Antibodies Raised against a Synthetic Peptide.

Authors:  J Wellink; M Jaegle; R Goldbach
Journal:  J Virol       Date:  1987-01       Impact factor: 5.103

8.  Purification of cowpea mosaic virus RNA replication complex: Identification of a virus-encoded 110,000-dalton polypeptide responsible for RNA chain elongation.

Authors:  L Dorssers; S van der Krol; J van der Meer; A van Kammen; P Zabel
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

9.  Association of viral 126 kDa protein-containing X-bodies with nuclei in mosaic-diseased tobacco leaves.

Authors:  M M Wijdeveld; R W Goldbach; B J Verduin; L C van Loon
Journal:  Arch Virol       Date:  1989       Impact factor: 2.574

10.  Antibody-linked polymerase assay on protein blots: a novel method for identifying polymerases following SDS-polyacrylamide gel electrophoresis.

Authors:  J van der Meer; L Dorssers; P Zabel
Journal:  EMBO J       Date:  1983       Impact factor: 11.598
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