Literature DB >> 16789262

Detection of a Novel Protein Encoded by the Bottom-Component RNA of Cowpea Mosaic Virus, Using Antibodies Raised against a Synthetic Peptide.

J Wellink1, M Jaegle, R Goldbach.   

Abstract

A peptide was synthesized that corresponded to a sequence in the cowpea mosaic virus bottom-component RNA-encoded 200-kilodalton polyprotein showing homology to the picornaviral 3C proteases. By injecting a rabbit with this peptide, antibodies were obtained that allowed the detection of a novel viral protein derived from the 200-kilodalton polyprotein. This protein, which had a size of 24 kilodaltons was found in both infected cowpea leaves and cowpea protoplasts.

Entities:  

Year:  1987        PMID: 16789262      PMCID: PMC255253     

Source DB:  PubMed          Journal:  J Virol        ISSN: 0022-538X            Impact factor:   5.103


  18 in total

1.  Protease required for processing picornaviral coat protein resides in the viral replicase gene.

Authors:  A C Palmenberg; M A Pallansch; R R Rueckert
Journal:  J Virol       Date:  1979-12       Impact factor: 5.103

2.  Expression of bottom component RNA of cowpea mosaic virus in cowpea protoplasts.

Authors:  G Rezelman; R Goldbach; A Van Kammen
Journal:  J Virol       Date:  1980-11       Impact factor: 5.103

3.  Comparison of different methods for localizing antigenic regions in histone H2A.

Authors:  S Muller; S Plaue; M Couppez; M H Van Regenmortel
Journal:  Mol Immunol       Date:  1986-06       Impact factor: 4.407

4.  Evidence for intramolecular self-cleavage of picornaviral replicase precursors.

Authors:  A C Palmenberg; R R Rueckert
Journal:  J Virol       Date:  1982-01       Impact factor: 5.103

5.  Comparison of in vivo and in vitro translation of cowpea mosaic virus RNAs.

Authors:  R W Goldbach; J G Schilthuis; G Rezelman
Journal:  Biochem Biophys Res Commun       Date:  1981-03-16       Impact factor: 3.575

6.  Expression of Middle-Component RNA of Cowpea Mosaic Virus: In Vitro Generation of a Precursor to Both Capsid Proteins by a Bottom-Component RNA-Encoded Protease from Infected Cells.

Authors:  H Franssen; R Goldbach; M Broekhuijsen; M Moerman; A van Kammen
Journal:  J Virol       Date:  1982-01       Impact factor: 5.103

7.  Purification of cowpea mosaic virus RNA replication complex: Identification of a virus-encoded 110,000-dalton polypeptide responsible for RNA chain elongation.

Authors:  L Dorssers; S van der Krol; J van der Meer; A van Kammen; P Zabel
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

8.  Similarity in gene organization and homology between proteins of animal picornaviruses and a plant comovirus suggest common ancestry of these virus families.

Authors:  P Argos; G Kamer; M J Nicklin; E Wimmer
Journal:  Nucleic Acids Res       Date:  1984-09-25       Impact factor: 16.971

9.  The nucleotide sequence of cowpea mosaic virus B RNA.

Authors:  G P Lomonossoff; M Shanks
Journal:  EMBO J       Date:  1983       Impact factor: 11.598

10.  Homologous sequences in non-structural proteins from cowpea mosaic virus and picornaviruses.

Authors:  H Franssen; J Leunissen; R Goldbach; G Lomonossoff; D Zimmern
Journal:  EMBO J       Date:  1984-04       Impact factor: 11.598

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  1 in total

1.  Polypeptide synthesis induced in Nicotiana clevelandii protoplasts by infection with raspberry ringspot nepovirus.

Authors:  O Acosta; M A Mayo
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

  1 in total

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