Phosphonoacetic Acid inhibition of frog virus 3 replication.

Phosphonoacetic acid at concentrations above 200 mug/ml inhibited the replication of frog virus 3 in BHK cells. The inhibition of viral DNA replication observed in these cells was reversible and correlated with the inhibition of the virus-induced DNA polymerase activity in an in vitro assay. The synthesis of frog virus 3-induced late or gamma polypeptides was also inhibited by phosphonoacetic acid, although the early (alpha and beta) polypeptides were unaffected.