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Literature DB >> 16782785

Channel activity of OmpF monitored in nano-BLMs.

Eva K Schmitt¹, Maarten Vrouenraets, Claudia Steinem.

Abstract

Free-standing lipid bilayer membranes can be formed on small apertures (60 nm diameter) on highly ordered porous alumina substrates. The formation process of the membranes on a 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol submonolayer was followed by impedance spectroscopy. After lipid bilayers had thinned, the reconstitution and ionic conducting properties of the outer membrane protein OmpF of E. coli were monitored using single-channel recordings. The characteristic conductance states of the three monomers, fast kinetics, and subconductance states were observed. Blockade of the ion flow as a result of interaction of the antibiotic ampicillin with the protein was verified, indicating the full functionality of the protein channel in nanometer-scale bilayer membranes.

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Year: 2006 PMID： 16782785 PMCID： PMC1557549 DOI： 10.1529/biophysj.106.083592

Source DB: PubMed Journal: Biophys J ISSN： 0006-3495 Impact factor: 4.033

40 in total

1. Whole cell patch clamp recording performed on a planar glass chip.

Authors: Niels Fertig; Robert H Blick; Jan C Behrends
Journal: Biophys J Date: 2002-06 Impact factor: 4.033

2. Alteration of pore properties of Escherichia coli OmpF induced by mutation of key residues in anti-loop 3 region.

Authors: Jérôme Bredin; Nathalie Saint; Monique Malléa; Emmanuelle Dé; Gérard Molle; Jean-Marie Pagès; Valérie Simonet
Journal: Biochem J Date: 2002-05-01 Impact factor: 3.857

3. Complex inhibition of OmpF and OmpC bacterial porins by polyamines.

Authors: R Iyer; A H Delcour
Journal: J Biol Chem Date: 1997-07-25 Impact factor: 5.157

4. Permeation properties of an engineered bacterial OmpF porin containing the EEEE-locus of Ca2+ channels.

Authors: Henk Miedema; Anita Meter-Arkema; Jenny Wierenga; John Tang; Bob Eisenberg; Wolfgang Nonner; Hans Hektor; Dirk Gillespie; Wim Meijberg
Journal: Biophys J Date: 2004-08-23 Impact factor: 4.033

5. Structural and functional characterization of OmpF porin mutants selected for larger pore size. II. Functional characterization.

Authors: N Saint; K L Lou; C Widmer; M Luckey; T Schirmer; J P Rosenbusch
Journal: J Biol Chem Date: 1996-08-23 Impact factor: 5.157

6. Glutamate receptor incorporated in a mixed hybrid bilayer lipid membrane array, as a sensing element of a biosensor working under flowing conditions.

Authors: Gabriele Favero; Luigi Campanella; Stefano Cavallo; Andrea D'Annibale; Maurizio Perrella; Elisabetta Mattei; Tommaso Ferri
Journal: J Am Chem Soc Date: 2005-06-08 Impact factor: 15.419

7. Fast and slow kinetics of porin channels from Escherichia coli reconstituted into giant liposomes and studied by patch-clamp.

Authors: C Berrier; A Coulombe; C Houssin; A Ghazi
Journal: FEBS Lett Date: 1992-07-20 Impact factor: 4.124

8. Membrane on a chip: a functional tethered lipid bilayer membrane on silicon oxide surfaces.

Authors: Vladimir Atanasov; Nikolaus Knorr; Randolph S Duran; Sven Ingebrandt; Andreas Offenhäusser; Wolfgang Knoll; Ingo Köper
Journal: Biophys J Date: 2005-09 Impact factor: 4.033

9. Ions and counterions in a biological channel: a molecular dynamics simulation of OmpF porin from Escherichia coli in an explicit membrane with 1 M KCl aqueous salt solution.

Authors: Wonpil Im; Benoît Roux
Journal: J Mol Biol Date: 2002-06-21 Impact factor: 5.469

10. Impedance analysis and single-channel recordings on nano-black lipid membranes based on porous alumina.

Authors: Winfried Römer; Claudia Steinem
Journal: Biophys J Date: 2004-02 Impact factor: 4.033

9 in total

Review 1. Applications of biological pores in nanomedicine, sensing, and nanoelectronics.

Authors: Sheereen Majd; Erik C Yusko; Yazan N Billeh; Michael X Macrae; Jerry Yang; Michael Mayer
Journal: Curr Opin Biotechnol Date: 2010-06-18 Impact factor: 9.740

2. Fractional polymerization of a suspended planar bilayer creates a fluid, highly stable membrane for ion channel recordings.

Authors: Benjamin A Heitz; Ian W Jones; Henry K Hall; Craig A Aspinwall; S Scott Saavedra
Journal: J Am Chem Soc Date: 2010-05-26 Impact factor: 15.419

Channel activity of OmpF monitored in nano-BLMs.

1. Whole cell patch clamp recording performed on a planar glass chip.

2. Alteration of pore properties of Escherichia coli OmpF induced by mutation of key residues in anti-loop 3 region.

3. Complex inhibition of OmpF and OmpC bacterial porins by polyamines.

4. Permeation properties of an engineered bacterial OmpF porin containing the EEEE-locus of Ca2+ channels.

5. Structural and functional characterization of OmpF porin mutants selected for larger pore size. II. Functional characterization.

6. Glutamate receptor incorporated in a mixed hybrid bilayer lipid membrane array, as a sensing element of a biosensor working under flowing conditions.

7. Fast and slow kinetics of porin channels from Escherichia coli reconstituted into giant liposomes and studied by patch-clamp.

8. Membrane on a chip: a functional tethered lipid bilayer membrane on silicon oxide surfaces.

9. Ions and counterions in a biological channel: a molecular dynamics simulation of OmpF porin from Escherichia coli in an explicit membrane with 1 M KCl aqueous salt solution.

10. Impedance analysis and single-channel recordings on nano-black lipid membranes based on porous alumina.

Review 1. Applications of biological pores in nanomedicine, sensing, and nanoelectronics.

2. Fractional polymerization of a suspended planar bilayer creates a fluid, highly stable membrane for ion channel recordings.

3. The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes.

4. Controlled delivery of proteins into bilayer lipid membranes on chip.

5. The Whole Is Bigger than the Sum of Its Parts: Drug Transport in the Context of Two Membranes with Active Efflux.

6. Modulation of the conductance of a 2,2'-bipyridine-functionalized peptidic ion channel by Ni2+.

7. Enhanced long-term stability for single ion channel recordings using suspended poly(lipid) bilayers.

8. Reconstitution of the membrane protein OmpF into biomimetic block copolymer-phospholipid hybrid membranes.

9. Structural basis for chitin acquisition by marine Vibrio species.