BACKGROUND: The purpose of this study was to develop a reproducible murine model of fetal scarless wound healing. METHODS: One-millimeter excisional wounds were made in fetal skin at gestational days 16.5 (E17) and 18.5 (E19) (term = day 21.5, or E22) and marked with India ink. Fetal mortality was less than 30 percent in E17 fetuses and 0 percent in E19 fetuses. Control postnatal 2-mm open wounds were made in 3-week-old mice. RESULTS: At 48 hours, E17 skin wounds had healed completely. E19 skin wounds also healed but were marked by skin irregularity at the wound site. Histologically, E17 wounds had fine reticular collagen architecture by trichrome staining and hair follicle regeneration. In contrast, E19 wounds healed with collagen deposition and scar formation and no hair follicle regeneration. CONCLUSIONS: The authors have developed a reliable mouse model of fetal scarless repair to help elucidate the mechanism of scarless wound healing to take advantage of genetically modified animals. The knowledge gained may be used to manipulate scarring in the adult to produce a more fetal-like wound.
BACKGROUND: The purpose of this study was to develop a reproducible murine model of fetal scarless wound healing. METHODS: One-millimeter excisional wounds were made in fetal skin at gestational days 16.5 (E17) and 18.5 (E19) (term = day 21.5, or E22) and marked with India ink. Fetal mortality was less than 30 percent in E17 fetuses and 0 percent in E19 fetuses. Control postnatal 2-mm open wounds were made in 3-week-old mice. RESULTS: At 48 hours, E17 skin wounds had healed completely. E19 skin wounds also healed but were marked by skin irregularity at the wound site. Histologically, E17 wounds had fine reticular collagen architecture by trichrome staining and hair follicle regeneration. In contrast, E19 wounds healed with collagen deposition and scar formation and no hair follicle regeneration. CONCLUSIONS: The authors have developed a reliable mouse model of fetal scarless repair to help elucidate the mechanism of scarless wound healing to take advantage of genetically modified animals. The knowledge gained may be used to manipulate scarring in the adult to produce a more fetal-like wound.
Authors: Michael Sung-Min Hu; Robert C Rennert; Adrian McArdle; Michael T Chung; Graham G Walmsley; Michael T Longaker; H Peter Lorenz Journal: Adv Wound Care (New Rochelle) Date: 2014-04-01 Impact factor: 4.730
Authors: Michael S Hu; Mimi R Borrelli; Michael Januszyk; Anna Luan; Samir Malhotra; Graham G Walmsley; Wan Xing Hong; Ruth Tevlin; Geoffrey C Gurtner; Michael T Longaker; Hermann P Lorenz Journal: Adv Wound Care (New Rochelle) Date: 2018-01-01 Impact factor: 4.730
Authors: Michael S Hu; Zeshaan N Maan; Jen-Chieh Wu; Robert C Rennert; Wan Xing Hong; Tiffany S Lai; Alexander T M Cheung; Graham G Walmsley; Michael T Chung; Adrian McArdle; Michael T Longaker; H Peter Lorenz Journal: Ann Biomed Eng Date: 2014-05-01 Impact factor: 3.934
Authors: Antoine L Carre; Barrett J Larson; Joseph A Knowles; Kenichiro Kawai; Michael T Longaker; H Peter Lorenz Journal: Ann Plast Surg Date: 2012-07 Impact factor: 1.539
Authors: Clement D Marshall; Michael S Hu; Tripp Leavitt; Leandra A Barnes; H Peter Lorenz; Michael T Longaker Journal: Adv Wound Care (New Rochelle) Date: 2018-02-01 Impact factor: 4.730
Authors: Nick L Occleston; Anthony D Metcalfe; Adam Boanas; Nicholas J Burgoyne; Kerry Nield; Sharon O'Kane; Mark W J Ferguson Journal: Dermatol Res Pract Date: 2010-08-03
Authors: Bindi Naik-Mathuria; Andre N Gay; Ling Yu; Jean E Hsu; C Wayne Smith; Oluyinka O Olutoye Journal: J Pediatr Surg Date: 2008-04 Impact factor: 2.545