Evaluation of the viability of pathogenic filamentous fungi after prolonged storage in sterile water and review of recent published studies on storage methods.

We have evaluated the survival and potential morphological alterations of 45 species of pathogenic filamentous fungi that had been stored in sterile water following Castellani's method in the National Collection of Pathogenic Fungi (NCPF). Storage duration varied from 2 months to over 21 years. Ninety percent of stored organisms were shown to be viable. Viability was largely independent of the duration of storage, but did apparently vary to some degree in an organism-specific manner. In addition, certain fungi were shown to have undergone morphological alterations during storage, and exhibited significant degrees of pleomorphism upon revival. This was especially marked for several isolates of dermatophytes, where storage resulted in loss of recognisable colonial features, and overproduction of sterile mycelium with aberrant or no conidia. These findings suggest that while Castellani's method remains an easy and inexpensive method for long-term preservation of most fungi, water storage should be supplemented by a second storage method to increase the chances of retaining both viability and morphological stability over long periods.