Literature DB >> 16754981

Crystallization and preliminary X-ray diffraction analysis of a tRNASer acceptor-stem microhelix.

Charlotte Förster1, Norbert Krauss, Arnd B E Brauer, Karol Szkaradkiewicz, Svenja Brode, Klaus Hennig, Jens P Fürste, Markus Perbandt, Christian Betzel, Volker A Erdmann.   

Abstract

In order to understand elongator tRNA(Ser) and suppressor tRNA(Sec) identity elements, the respective acceptor-stem helices have been synthesized and crystallized in order to analyse and compare their structures in detail at high resolution. The synthesis, crystallization and preliminary X-ray diffraction results for a seven-base-pair tRNA(Ser) acceptor-stem helix are presented here. Diffraction data were collected to 1.8 A, applying synchrotron radiation and cryogenic cooling. The crystals belong to the monoclinic space group C2, with unit-cell parameters a = 36.14, b = 38.96, c = 30.81 A, beta = 110.69 degrees .

Entities:  

Mesh：

Substances：

Year:  2006        PMID： 16754981      PMCID： PMC2243086          DOI： 10.1107/S1744309106016472

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  17 in total

1.  Identification and characterisation of the selenocysteine-specific translation factor SelB from the archaeon Methanococcus jannaschii.

Authors:  M Rother; R Wilting; S Commans; A Böck
Journal:  J Mol Biol       Date:  2000-06-02       Impact factor: 5.469

Review 2.  Incorporation of nonnatural amino acids into proteins.

Authors:  Tamara L Hendrickson; Valérie de Crécy-Lagard; Paul Schimmel
Journal:  Annu Rev Biochem       Date:  2004       Impact factor: 23.643

3.  Selenocysteine synthase from Escherichia coli. Nucleotide sequence of the gene (selA) and purification of the protein.

Authors:  K Forchhammer; W Leinfelder; K Boesmiller; B Veprek; A Böck
Journal:  J Biol Chem       Date:  1991-04-05       Impact factor: 5.157

4.  Identification of a novel translation factor necessary for the incorporation of selenocysteine into protein.

Authors:  K Forchhammer; W Leinfelder; A Böck
Journal:  Nature       Date:  1989-11-23       Impact factor: 49.962

5.  Gene for a novel tRNA species that accepts L-serine and cotranslationally inserts selenocysteine.

Authors:  W Leinfelder; E Zehelein; M A Mandrand-Berthelot; A Böck
Journal:  Nature       Date:  1988-02-25       Impact factor: 49.962

6.  Solvent content of protein crystals.

Authors:  B W Matthews
Journal:  J Mol Biol       Date:  1968-04-28       Impact factor: 5.469

Review 7.  Aminoacyl tRNA synthetases: general scheme of structure-function relationships in the polypeptides and recognition of transfer RNAs.

Authors:  P Schimmel
Journal:  Annu Rev Biochem       Date:  1987       Impact factor: 23.643

8.  Interaction of a selenocysteine-incorporating tRNA with elongation factor Tu from E.coli.

Authors:  C Förster; G Ott; K Forchhammer; M Sprinzl
Journal:  Nucleic Acids Res       Date:  1990-02-11       Impact factor: 16.971

9.  Partition of tRNA synthetases into two classes based on mutually exclusive sets of sequence motifs.

Authors:  G Eriani; M Delarue; O Poch; J Gangloff; D Moras
Journal:  Nature       Date:  1990-09-13       Impact factor: 49.962

10.  Selenocysteine synthase from Escherichia coli. Analysis of the reaction sequence.

Authors:  K Forchhammer; A Böck
Journal:  J Biol Chem       Date:  1991-04-05       Impact factor: 5.157
View more
  2 in total

1.  Crystallization and preliminary X-ray diffraction analysis of an Escherichia coli tRNA(Gly) acceptor-stem microhelix.

Authors:  Charlotte Förster; Markus Perbandt; Arnd B E Brauer; Svenja Brode; Jens P Fürste; Christian Betzel; Volker A Erdmann
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-12-22

2.  Human tRNA(Gly) acceptor-stem microhelix: crystallization and preliminary X-ray diffraction analysis at 1.2 A resolution.

Authors:  Charlotte Förster; Karol Szkaradkiewicz; Markus Perbandt; Arnd B E Brauer; Tordis Borowski; Jens P Fürste; Christian Betzel; Volker A Erdmann
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-09-19
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.