BACKGROUND: In age related macular degeneration and inherited dystrophies, preservation of retinal ganglion cells has been demonstrated. This finding has led to the development of various models of subretinal or epiretinal implant in order to restore vision. This study addresses the development of a polyimide subretinal electrode platform in the dystrophic P23H rat in vivo. METHODS: A technique was developed for implanting a subretinal electrode into the subretinal space and stabilising the distal extremity of the cabling on the rat cranium in order to allow future electrical stimulations of the retina. RESULTS: In vivo imaging of the retina with the scanning laser ophthalmoscope demonstrated reabsorption of the surgically induced retinal detachment and the absence of major tissue reactions. These in vivo observations were confirmed by retinal histology. The extraocular fixation system on the rat cranium was effective in stabilising the distal connector for in vivo stimulation. CONCLUSION: This study demonstrates that a retinal implant can be introduced into the subretinal space of a dystrophic rat with a stable external connection for repeatable electrical measurements and stimulation. This in vivo model should therefore allow us to evaluate the safety and efficacy of electrical stimulations on dystrophic retina.
BACKGROUND: In age related macular degeneration and inherited dystrophies, preservation of retinal ganglion cells has been demonstrated. This finding has led to the development of various models of subretinal or epiretinal implant in order to restore vision. This study addresses the development of a polyimide subretinal electrode platform in the dystrophicP23Hrat in vivo. METHODS: A technique was developed for implanting a subretinal electrode into the subretinal space and stabilising the distal extremity of the cabling on the rat cranium in order to allow future electrical stimulations of the retina. RESULTS: In vivo imaging of the retina with the scanning laser ophthalmoscope demonstrated reabsorption of the surgically induced retinal detachment and the absence of major tissue reactions. These in vivo observations were confirmed by retinal histology. The extraocular fixation system on the rat cranium was effective in stabilising the distal connector for in vivo stimulation. CONCLUSION: This study demonstrates that a retinal implant can be introduced into the subretinal space of a dystrophicrat with a stable external connection for repeatable electrical measurements and stimulation. This in vivo model should therefore allow us to evaluate the safety and efficacy of electrical stimulations on dystrophic retina.
Authors: Hugo Hämmerle; Karin Kobuch; Konrad Kohler; Wilfried Nisch; Helmut Sachs; Martin Stelzle Journal: Biomaterials Date: 2002-02 Impact factor: 12.479
Authors: Yves A Kerdraon; John A Downie; Gregg J Suaning; Malcolm R Capon; Minas T Coroneo; Nigel H Lovell Journal: Clin Exp Ophthalmol Date: 2002-02 Impact factor: 4.207
Authors: A Y Chow; M T Pardue; V Y Chow; G A Peyman; C Liang; J I Perlman; N S Peachey Journal: IEEE Trans Neural Syst Rehabil Eng Date: 2001-03 Impact factor: 3.802
Authors: H N Schwahn; F Gekeler; K Kohler; K Kobuch; H G Sachs; F Schulmeyer; W Jakob; V P Gabel; E Zrenner Journal: Graefes Arch Clin Exp Ophthalmol Date: 2001-12 Impact factor: 3.117
Authors: P M D'Cruz; D Yasumura; J Weir; M T Matthes; H Abderrahim; M M LaVail; D Vollrath Journal: Hum Mol Genet Date: 2000-03-01 Impact factor: 6.150
Authors: G Banisadr; F Quéraud-Lesaux; M C Boutterin; D Pélaprat; B Zalc; W Rostène; F Haour; S Mélik Parsadaniantz Journal: J Neurochem Date: 2002-04 Impact factor: 5.372
Authors: Dimiter R Bertschinger; Evgueny Beknazar; Manuel Simonutti; Avinoam B Safran; José A Sahel; Serge G Rosolen; Serge Picaud; Joel Salzmann Journal: Graefes Arch Clin Exp Ophthalmol Date: 2008-08-16 Impact factor: 3.117
Authors: Matthew M LaVail; Shimpei Nishikawa; Roy H Steinberg; Muna I Naash; Jacque L Duncan; Nikolaus Trautmann; Michael T Matthes; Douglas Yasumura; Cathy Lau-Villacorta; Jeannie Chen; Ward M Peterson; Haidong Yang; John G Flannery Journal: Exp Eye Res Date: 2017-11-06 Impact factor: 3.467
Authors: Chris Sekirnjak; Lauren H Jepson; Pawel Hottowy; Alexander Sher; Wladyslaw Dabrowski; A M Litke; E J Chichilnisky Journal: J Neurophysiol Date: 2011-03-09 Impact factor: 2.714
Authors: Chris Sekirnjak; Clare Hulse; Lauren H Jepson; Pawel Hottowy; Alexander Sher; Wladyslaw Dabrowski; A M Litke; E J Chichilnisky Journal: J Neurophysiol Date: 2009-09-02 Impact factor: 2.714
Authors: Jorge V Sotoca; Juan C Alvarado; Verónica Fuentes-Santamaría; Juan R Martinez-Galan; Elena Caminos Journal: Front Neurosci Date: 2014-09-17 Impact factor: 4.677
Authors: Anil Vaidya; Elio Borgonovi; Rod S Taylor; José-Alain Sahel; Stanislao Rizzo; Paulo Eduardo Stanga; Amit Kukreja; Peter Walter Journal: BMC Ophthalmol Date: 2014-04-14 Impact factor: 2.209